Throughput limits

Special Problems Reflection Losses Result in Low Optical Throughput Limiting Signal to Noise Highly Susceptible to Airborne Noise and Build-inq Vibrations... 

Operating limitations (bottlenecks)—there could be a throughput limitation on reactants to one of the processes (e.g., available pressure head). 

In many projects, the throughput limit for the confirmation assay is significantly lower than the number of actives from the primary HTS, and in these cases, a Top X approach based on a preselected threshold has the obvious disadvantage of failing to consider a potentially large number of true actives, leading to an artificially high... 

Lastly, every research project presents a unique combination of parameters (target, assay methodology, hit rate, throughput limit, and so forth) and project team requirements for interesting actives (physiochemical properties, novelty, and so forth). This suggests that an optimal selection scheme should be easily tailored to meet these unique needs, which is certainly not true of a simple Top X approach. 

The confirmed actives selected from each of the three example projects shown are at varying stages of progression through the secondary assay process. The peptide hydrolase example is the most advanced and so has the most information available. The throughput limit for secondary assays in that case was just 500 molecules, and... 

Generation of organic solvent waste Short analysis time and high throughput Limited portability. Confined to the laboratory Applicable to early-warning and on-site monitoring Qualified personnel required Equipment expensive... 

The Quattro system allows recording of signals in real time, corresponding to channel function. However, the higher throughput limits the complexity of the protocols that can be used, as well as constrains the number of compound additions to a two-step discontinuous clamp process, as with the first generation system. 

Offset Lithography High throughput. Limited in thickness Not expected to be a major... 

To address the low throughput limitation associated with most screening methods, various fluorescence-activated cell sorting (FACS) based screening methods have been developed. Unlike the above mentioned screening methods, FACS can analyze and sort up to 100,000 cells per second in a quantitative manner... 

Cellulose acetate Very low non-specific adsorption (non-fouling) High flow rates and total throughputs Limited pH compatibility Not dry autoclavable... 

Processes that have throughput limitations that could benefit from additional heat transfer without adversely affecting product quality, and... 

Extended furnace life by overcoming combustion air throughput limitations caused by plugged checkers or failing recuperator ... 

Potentiometry is a further technique used to measure aqueous solubility in discovery, although throughput limitations mean that this technique is used most often later in the LO stage. Potentiometric approaches specifically measure intrinsic solubility, which is the solubility of the neutral form of the molecule [38],... 

Tissue-based systems evaluation of transport mechanism segmental absorption evaluation evaluation of absorption enhancing strategies other tissues (nasal, buccal) comparison between species possible presence of nerves (e.g., local control, crypt-villus axis) transporters and enzymes as in vivo limited database viability of tissue low throughput limited access to (human) tissue... 

---