The Carbohydrate-Recognition Domain

The precise chemical interactions between an adhesin and its receptor are also important. For example, direct- and water-mediated hydrogen bonds are the most important interactions within the carbohydrate-recognition domain in carbohydrate-binding adhesins on the host cell surface (Weis and Drickamer, 1996). Nonpolar van der Waals interactions and hydrophobic "stacking of the receptor oligosaccharide rings with aromatic amino acid side chains of the bacterial adhesin protein also contribute to oligosaccharide-protein interactions. X-ray structural... 

Figure 13.2 The crystal structures of (a) concanavalin A and (b) the carbohydrate recognition domain of galectin-3. Residues in the carbohydrate binding sites are black.

McGreal EP, Rosas M, Brown GD, Zamze S, Wong SYC, Gordon S, Martinez-Pomares L, Taylor PR. The carbohydrate-recognition domain of Dectin-2 is a C-type lectin with specificity for high mannose. Glycobiology 2006 16 422-430. 

Pichierri, F, Matsuo, Y, Effect of protonation of the A-acetyl neuraminic acid residue of sialyl Lewis a molecular orbital study with insights into its binding properties toward the carbohydrate recognition domain of E-selectin, Bioorg. Med. Chem. Lett., 10, 2751-2757, 2002. 

Whereas a wealth of structural information derived from x-ray and NMR spectroscopy[9, 11, 12], is available for Types 1 and III AFPs, very little is known about the three dimensional structure of the largest, Type II AFP and Type IV. A three dimensional structure of Type II antifreeze protein was recently proposed using comparative modeling[13]. This structure was derived using a sequence similarity to the carbohydrate recognition domain (CDR) of... 

S. Rabbani, X. H. Jiang, O. Schwardt and B. Ernst, Expression of the carbohydrate recognition domain of EimH and development of a competitive binding assay, Awfl/. Biochem, 2010, 407, 188-195. 

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