Testosterone-imprinted polymers

Metal nanoparticles have also been included into MIPs. Such particles can be used, for example, as nanoantennae for the enhancement of electromagnetic waves (plasmonic enhancement). It has been shown by He et al. [122] that a thin layer (20-120 nm) of testosterone-imprinted silica could be synthesized around 350 nm silver particles in a controlled way. The composite material showed specific binding of the testosterone target. Matsui et al. [123] reported a molecularly imprinted polymer with immobilized Au nanoparticles as a sensing material for spectrometry. The sensing mechanism is based on the variable proximity of the Au nanoparticles... 

Bui BTS, Merlier F, Haupt K (2010) Toward the use of a molecularly imprinted polymer in doping analysis selective preconcentration and analysis of testosterone and epitestosterone in human urine. Anal Chem 82(11) 4420-4427... 

Other SPME-IMS methods that have been reported for application to pharmaceutical or related samples include those for analysis of ephedrine in urine, meth-amphetamines in human serum, and captopril in human plasma and pharmaceutical preparations. In a method similar to SPME-IMS, testosterone was collected with a molecular imprinted polymer from urine and desorbed into an IMS. The method was validated with HPLC and determined to have a detection limit of 0.9 ng/mL with a linear dynamic range from 10 to 250 ng/mL. ... 

Cheong, S.-H. Rachkov, A.E. Park, J.-K. Yano, K. Karube, I. Synthesis and binding properties of a noncovalent molecularly imprinted testosterone-specific polymer. J. Polym. Chem., A. Polym. Chem. 1998, 36, 1725-1732. 

The molecularly imprinted copolymer of 2-hydroxyethyl methacrylate (HEMA) and p-CyD-coupled HEMA was synthesized in chloroform to study its interaction with a pair of steroids cholesterol and testosterone [42]. The molecularly imprinted copolymer was found to absorb the print molecule several times better than an imprinted poly HEMA. Asanuma et al. [44] concluded that cholesterol binding by the HEMA polymer was not due to CyD inclusion because no inclusion of CyD to cholesterol can form in chloroform. Another strategy developed for imprinting using CyD elements is the vinyl CyD (Table 1 and 10.3(A)), which has been successfully applied for imprinting antibodies, oligo-peptides [45], and vancomycin [46[. 

McNiven et al. [22] first demonstrated this guest directed chemical modification strategy. In their studies, an MAA/EGDMA polymer imprinted with testosterone (Fig. 3) was partially esterified with methyl iodide (Mel) and 1,8-diazabicyclo(5.4.0) undec-7-ene (DBU) both in the presence and absence of the... 

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