Test methods antimicrobial agent determining

ASTM E 2180 Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) in Polymeric or Hydrophobic Materials ... 

Test methods to demonstrate the efficacy of additives, such as antimicrobial agents, should be used to determine whether such additives remain effective and unchanged throughout the projected shelf-life. 

ASTM E2149-10 Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. 

The agar diffusion method (Kirby—Bauer) is also sometimes used for the evaluation of antibacterial activity of textiles. This is a relatively quick and easily executed semiquantitative method to determine antibacterial activity of diffusible antimicrobial agents on treated textile material. The bacteria are grown in nutrient broth medium and after appropriate dilution (e.g., lOOx) from the culture, test organisms are swabbed over the surface of agar plates. Ten-millimetre-diameter disks of the test fabric and control fabric are then gently pressed onto the surface of the plate. The plates are then incubated at 37 °C for 18—24 h. The antibacterial activity of the fabrics is demonstrated by the diameter of the zone of inhibition in comparison to the control textile sample. 

ASTM E1427-00. Standard Guide for seleeting test methods to determine the effectiveness of antimicrobial agents and other chemicals for the... 

American Society for Testing and Materials. ASTM E2149-10 standard test method for determining the antimicrobial activity of immobilized antimicrobial agents under dynamic contact conditions. Pennsylvania ASTM IntemationaL PA 2001. 

EUCAST (2000), Terminology relating to methods for the determination of snscepti-bility of bacteria to antimicrobial agents , European Committee on Antimicrobial Susceptibility Testing Definitive document E.Def 1.2 May 2000 http //www.srga. org/Eucastwt/eucastdefinitions.htm. 

Antimicrobial resistance of major consequence may be detected, e.g., the test is very useful in detecting glycopeptide-resistant enterococci and glycopeptide-intermediate Staphylococcus aureus and slow-growing pathogens such as Mycobacterium tuberculosis. Furthermore, it can be used for detecting extended spectrum P-lactamases. In conclusion, the E-test is a simple, accurate and reliable method to determine the MIC for a wide spectrum of infectious agents [33, 34]. 

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