Antimicrobial test methods

Table 15.1 Antimicrobial test methods used with textiles...

Table 15.2 Comparison of main antimicrobial test methods according to Dring ... 

Antimicrobial test methods should also utilize a reliable supply of standardized microorganisms. 

Cowen R. A. Steiger B. (1976) Antimicrobial activity—a critical review of test methods of preservative efficiency. J Soc Cosmet Chem, 27, 467-481. 

Sterility testing Methods Antimicrobial agents 8 Further reading... 

In 1998, a CEFIC working group composed of industry experts from EPAS (European Producers of Antimicrobial Substances) and EPFP (European Producers of Formulated Preservatives) compiled a listing of over 200 relevant efficacy testing methods and around 100 of these were critically analysed in... 

The purpose of this example is to demonstrate the efficacy of a silver composition of the present invention against the bacteria that cause tuberculosis. This example describes the procedures for evaluation of the present invention for tuberculocidal efficacy. The methodology is based on the Tuberculocidal Activity Test Method as accepted by the EPAon Dec. 11, 1985. [Refer to United States Environmental Protection Agency, 1986. Office of Pesticides and Toxic Substances. Data Call-In Notice for Tubercuolocidal Effectiveness Data for All Antimicrobial Pesticides with Tuberculocidal Claims. (Received Jun. 13, 1986). 

Microbial Test Methods The selected microbial test methods determine specific sampling and analytical procedures. When the product has a potential antimicrobial effect and/or preservative, the spread technique on microbial test plates must be validated. In addition, the personnel performing the analytical techniques have to be qualified and adequately trained for this purpose [6],... 

Antimicrobial activity assessment of carpets test method 174... 

The thiophene polyine ( )-2[5-hept-5-en-l,3-diynyl)-thien-2-yl]-ethan-1,2-diol isolated from an ethanolic extract of the underground parts of Leuzea carthamoides DC. demonstrated significant antifungal activity [247], while the dichloromethane extract of the air-dried leaves of Chrysanthemum coronarium afforded N-isobutyl-6-(2-thiophenyl)-2,4-hexadienamide. Fig. (46) [248]. Antimicrobial tests by the agar well diffusion method indicated that it has low activity against Bacillus subtilis. Pseudomonas aeruginosa and Candida albicans, and is inactive against Staphylococcus aureus and Escherichia coli at a concentration of 20 pg/ml. 

Most clinical laboratories now employ agar dilution breakpoint testing methods. These are essentially truncated agar dilution MIC tests employing only a small range of antimicrobial concentrations around the critical susceptible/resistant cut off levels. 

The following sections will discuss some of the more important test methods in use for fabrics with durable press, flame-retardant, soil release, repellent, UV protective, antimicrobial, anti-insect, anti-felting, hand building and weighting finishes. 

Isquith, A. ]. and McCollum, C. J. "Surface kinetic test method for determining rate of kill by an antimicrobial solid Appl. Environ. Microbiol. 1978. 36 700-704. 

Most antimicrobial susceptibility tests used in the clinical laboratory are well characterized and have been standardized by the National Committee for Clinical Laboratory Standards (NCCLS). However, controversies still exist surrounding the exact test methods, interpretation and reporting of test results, and application of the results to the treatment of patients. Nevertheless, there are many investigations that show that the general antimicrobial susceptibility or resistance profile of an infecting organism correlates with clinical and/or microbiologic responses to therapy. 

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