TEMED

The most commonly used combination of chemicals to produce a polyacrylamide gel is acrylamide, bis acrylamide, buffer, ammonium persulfate, and tetramethylenediarnine (TEMED). TEMED and ammonium persulfate are catalysts to the polymerization reaction. The TEMED causes the persulfate to produce free radicals, causing polymerization. Because this is a free-radical driven reaction, the mixture of reagents must be degassed before it is used. The mixture polymerizes quickly after TEMED addition, so it should be poured into the gel-casting apparatus as quickly as possible. Once the gel is poured into a prepared form, a comb can be appHed to the top portion of the gel before polymerization occurs. This comb sets small indentations permanently into the top portion of the gel which can be used to load samples. If the comb is used, samples are then typically mixed with a heavier solution, such as glycerol, before the sample is appHed to the gel, to prevent the sample from dispersing into the reservoir buffer. [Pg.182]

V,7V,7V, 7V -Tetramethylethylenediamine (TMEDA, TEMED) [110-18-9] M 116.2, b 122°, d 1.175, n 1.4153, pK 5.90, pKj 9.14. Partially dried with molecular sieves (Linde type 4A), and distd in vacuum from butyl lithium. This treatment removes all traces of primary and secondary amines and water. [Hay, McCabe and Robb J Chem Soc, Faraday Trans 1 68 1 1972.] Or, dried with KOH pellets. Refluxed for 2h with one-sixth its weight of n-butyric anhydride (to remove primary and secondary amines) and fractionally distd. Refluxed with fresh KOH, and distd under nitrogen. [Cram and Wilson 7 Am C/iem Soc 85 1245 796i.] Also distd from sodium. [Pg.364]

Temes TA, Joss A (2006) Human pharmaceuticals, hormones and fragrances. The challenge of micropollutans in urban management. IWA, London, UK... [Pg.125]

Hummel D, Loffler D, Fink G, Temes TA (2006) Simultaneous determination of psychoactive drugs and their metabolites in aqueous matrices by liquid chromatography mass spectrometry. Environ Sci Technol 40 7321-7328... [Pg.225]

Joss A, Keller E, Alder AC, Gobel A, McArdell CS, Temes T, Slegrlst H (2005) Removal of pharmaceuticals and fragrances in biological wastewater treatment. Water Res 39(14) 3139-3152... [Pg.226]

Temes TA (1998) Occurrence of dmgs in German sewage treatment plants and rivers. Water Res 32 3245-3260... [Pg.226]

Carballa M, Omil F, Lema JM, Llompart M, Garcia-Jares C, Rodriguez I, Gomez M, Temes T... [Pg.226]

Sadezky A, Loffler D, Temes T (2008) Proposal of an environmental indicator and classification system of pharmaceutical product residues for environmental management KNAPPE project Knowledge and need assessment on pharmaceutical products in environmental waters . Contract n° 036864. Deliverable 1.2. http //www.knappe-eu.org/... [Pg.226]

Temes TA (2001) Analytical methods for the determination of pharmaceuticals in aqueous environmental samples. Trends Analyt Chem 20 419 34... [Pg.227]

Temes TA, Bonerz M, Herrmann N, Teiser B, Andersen HR (2007) Irrigation of treated wastewater in Braunschweig, Germany an option to remove pharmaceuticals and musk fragrances. Chemosphere 66 894-904... [Pg.228]

Wartenberg KE, Schmidt JM, Claassen J, Temes RE, Frontera JA, Ostapkovich N, Parra A, Connolly ES, Mayer SA. Impact of medical complications on outcome after subarachnoid hemorrhage. Crit Care Med 2006 34(3) 617-623. [Pg.190]

He takes the book and looks at it. I had no time at Northampton, and this little book is all I could bring with me. I can see the lad is disappointed, in the book and its small, rough woodcuts, and in me as its possessor. It s a printed book. There are hundreds the same, made by a press, a machine, and sold for a shilling or two. You could have many yourself, full of prayers and tales and great deeds. Not just a missal. I see his face, and laugh. I know. Most boys do not care for book-learning. Even Ned had to be tempted to his desk, when the sun was out over the Teme and he had a new horse to try. ... [Pg.333]

A monodentate palladium(II) complex trans-[Pd(py)2(H202)]2+ hydrolyzes Met-Aa amide bonds with a rate comparable with that promoted by [Pd(H20)3(0H)]+. Unlike Pd(H20)3(0H)]+, //chelated complex containing temed (A,A,AAA -tctramcthylcthylenediamine) hydrolyzes Met-Aa amide bonds with hydrolytic rate controlled by temed release. The action of the other two complexes, c -[Pd(ED-TA)C12] (EDTA = ethylene diaminetetraacetic acid) and cis-1,2-bis(2-formylglycinebenzene-sulfenyl)ethane Pd11 chloride differs from the action of similar complexes of U,v-[Pd(en)Cl2] and cw-[Pd(dtco-3-OH)Cl2] (dtco-3-OH = l,5-dithiacycooctan-3-ol).448... [Pg.592]

Johnson AC, Temes T, Williams RJ, Sumpter JP (2008) Assessing the concentrations of polar organic microcontaminants from point sources in the aquatic environment measure or model Environ Sci Technol 42(15) 5390-5399... [Pg.44]

Richardson SD, Temes TA (2011) Water analysis emerging contaminants and current issues. Anal Chem 83(12) 4614-4648... [Pg.45]

Temes TA, Andersen H, Gilberg D, Bonerz M (2002) Anal Chem 74 3498... [Pg.68]

Krogh KA, Bjorklund E, Loeffler D, Fink G, Halling-Sorensen B, Temes TA (2008) J Chromatogr A 1211 60... [Pg.69]

Temes TA, Kreckel P, Mueller J (1999) Behaviour and occurrence of estrogens in municipal sewage treatment plants - II. Aerobic batch experiments with activated sludge. Sci Total Environ 225 91-99... [Pg.107]

Andersen H, Siegrist H, Halling-Sorensen B, Temes TA (2003) Fate of estrogens in a municipal sewage treatment plant Environ Sci Technol 37 4021 1026... [Pg.107]

Joss A, Andersen H, Temes T, Richie PR, Siegrist H (2004) Removal of estrogens in municipal wastewater treatment under aerobic and anaerobic conditions consequences for plant optimization. Environ Sci Technol 38 3047-3055... [Pg.107]

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