Peptide Tags

Another popular assay format for kinase assays is the Lanthascreen. This format is a variation on the LANCE assay, but employs Tb as the cryptate. In this format N-terminally fluorescently tagged peptide substrate (acceptor) is phosphorylated by the kinase. Next, a phophospecific antibody which is labeled with terbium binds specifically to the phosphorylated product, placing the donor and acceptor in close proximity, generating a signal [25]. 

Nelson, R. W. Jarvik, J. W. Taillon, B. E. Tubbs, K. A. BIA/MS of epitope-tagged peptides directly from E. coli lysate Multiplex detection and protein identificatin at low-femtomole to subfemtomole levels. Anal. Chem. 1999, 71, 2858-2865. 

Thompson, A., Prescott, M., Chelebi, N., Smith, J., Brown, T., and Schmidt, G. (2007) Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates Synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS. Nucleic Acids Res. 35(4), e28. 

Ueno, A. Shimizu, T. Mihara, H. Hamasaki, K. and Pitchumani, K. (2002) Supramolecular Chemistry of Cyclodextrin-Peptide Hybrids Azobenzene-Tagged Peptides,/. Incl. Phenom. 44, 49-52. 

To discriminate between the fluorescence due to binding of the BODIPY-tagged peptide on the surface of the bacterial cells, that is accessible to quenching, from that given by the internalized peptide, that is not, add the impermeant quencher Trypan Blue at a Anal concentration of 1 mg/mL and incubate for 10 min at room temperature. 

Recognition of a specific amino acid sequence from a protein or a specific tag peptide sequence is expected to lead to variety of medical and materials applications. Whereas the recognition of a single amino acid residue by synthetic hosts has been reported previously, the artificial recognition of a sequence of two or more amino acid residues has been quite limited. There are only a few reports on the... 

Nucleotide tags Peptide tags Haloaromatic tags Secondary amine tags Isotopic tags Autotagging... 

Two or more samples are treated with isotopic reagents to tag them before they are pooled with equal protein amounts. The tags are chemically identical but differ in their masses due to isotopes in the reagents. In order to reduce complexity, proteins can be separated by either LC- or 2DE-techniques before they are enzymatically digested. MS based detection can be performed either with MAT,PT or ESI. Analysis of the MS spectra allows identification of the proteins present. Examination of the following MS/MS scans allows relative quantitation of the differently tagged peptides. The ratio corresponds to the amount of protein in the different samples. 

Nanokan Easy readout with radiofrequency tags Peptide library is relatively small ... 

NUCLEOTIDE TAGS PEPTIDE TAGS HALOAROMATIC TAGS SECONDARY AMINE TAGS ISOTOPIC TAGS AUTOTAGGING... 

In some algae, cpDNA encodes tmRNA, small RNA molecules that combine the functions of tRNA and mRNA and ate widespread in bacteria. These tmRNAs encode proteolysis-inducing tag-peptides, which bind to the 3 end of damaged mRNA molecules during translation, initiating proteolysis of the defective polypeptide. Such a fascinating mode of proteolysis is unknown elsewhere in eukaryotes. ... 

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