Stable isotope tagging

Tao WA, Aebersold R (2003) Advances in quantitative proteomics via stable isotope tagging and mass spectrometry. Curr Opin Biotechnol 14 110-118... 

Floey, M. R., Geieein, T. J., Martin, D., Aebersold, R. (2002). Advances in quantitative proteomics using stable isotope tags. Trends Biotechnd. 20, 23-29. 

Che FY, Biswas R, Fricker LD. Relative quantitation of peptides in wild-type and Cpe(fat/fat) mouse pituitary using stable isotopic tags and mass spectrometry. J. Mass Spectrom. 2005 40 227-237. Decaillot FM, Che FY, Fricker LD, Devi LA. Peptidomics of Cpefat/fat mouse hypothalamus and striatum effect of chronic morphine administration. J. Mol. Neurosci. 2006 28 277-284. Naggert JK, Fricker LK, Varlamov O, Nishina PM, Rouille Y, Steiner DF, Carroll RJ, Paigen BJ, Leiter EH. Hyperproinsuli-naemia in obese fat/fat mice associated with a carboxypepti-dase E mutation which reduces enzyme activity. Nat. Genet. 1995 10 135-142. 

Incorporation of a stable isotopic tag into proteins/peptides in metabolically active cells was first described to quantify protein abundance in yeast (43). Wild-type and mutant cell populations were grown in media containing the naturally abundant isotopes of nitrogen and enriched in 15N, respectively, followed by trypsin digestion and LC-ESI-MS/MS analysis to identify and quantify relative phosphopeptide levels in both populations (43). 

In another variant for quantitative proteome analysis by 2D PAGE, one could exploit the technique of stable isotope tagging. In this approach, the labeling strategy involves light/heavy forms of the same tagging molecule. An example of such an approach... 

Ehrenkranz, R.A., Gettner, P.A., NeDi, C.M., Sherwonit, E.A., Williams, J.E., Ting, B.T., and Janghorbani, M. (1991) Selenium absorption and retention by very-low-birth-weight infants studies with the extrinsic stable isotope tag Se. J. Pediatr. Gastroenterol. Nutr, 13, 125-133. 

The stable-isotope tags can be introduced into proteins or peptides by using different methods. These methods can be divided into the following categories ... 

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