SUVs — small unilamellar

Suv, small unilamellare vesicles luv, large unilamellare vesicles mlv, multilamellare vesicles mvv, multivesiculare vesicles (Fig. 4 from [1.34]). 

SUV = small unilamellar liposomes, LUV = large unilamellar liposomes. 

Figure 3 Design of a diepitope liposomal construct. Small unilamellar liposomes (PC/PG/Chol 55/25/50 molar ratio diameter 100nm) containing 10mol% of bromo-acetyl l,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine and 10mol% of the thiol-reactive lipopeptide adjuvant anchor Pam3CysAlaGly-Mal were reacted, at 25°C successively at pH 6.5, with the T-helper epitope QYI, derivatized with a C-linker at its N-terminus, followed at pH 9.0 by the B-epitope TPE derivatized with a CG linker at its N-terminus. Abbreviations PC, phosphatidylcholine PE, phosphatidylethanolamine SUV, small unilamellar vesicles. Source From Refs. 11, 20, 21.

Fig. 5. Types of liposomes SUV, small unilamellar vesicle LUV, large unilamellar vesicle MLV, multilamellar vesicle MW, multivesicular vesicle...

FIGURE 39.1 Approximate size ranges for the commonly used colloidal dmg delivery systems. SUV = small unilamellar vesicle LUV = large unilamellar vesicle MLV = large multilamellar vesicle MW = multivesicular vesicle. 

Abbreviations CCK, cholecystokinin RNase. ribonuclease G-protein, guanine nucleotide binding protein GPCR, G-protein-coupled receptor. SDS sodium dodecylsulfate, CTAH. hexadecyltrimethyl ammonium hydroxide DMPC. di-myristoylphosphatidylcholine DPPC, di-palmitoylphosphatldylcholine CMC, critical micellar concentration SUV, small unilamellar vesicles CD, circular dichroism NMR, nuclear magnetic resonance hs-DC, high sensitivity differential scanning calorimetry IR-ATR, infrared attenuated total reflection spectroscopy NOE, nuclear Overhauser effect MD, molecular dynamics DMSO, dimethylsulfoxide TFE, trifluoroethanol for abbreviations of peptides see tables land 2, and fig. 11. 

Abbreviations IgGl. immunoglobulin Gl KLH, keyhole limpet hemocyanin BSA, bovine serum albumin RNase A. ribonuclease A LDH. lactate dehydrogenase MHC, major histocompatibility complex LHRH, luteneizing hormone releasing hormone CCK. cholecystokinin VIP. vasoactive intestinal peptide TASP, template-assembled synthetic proteins MAPS, multiple antigen presenting system SUV, small unilamellar vesicle DCC. dicyclohexylcarbodiimide HOSu. N-hydroxysuccinimide Mal>. maleimido TFA, trifluoroacelic acid TFE. triiluoroethanol DMF, dimethylformamide DMSO. 

An excellent compromise in simplicity and value of information is found in the use of LUVs, which can be probed by various complementary methods such as fluorescence and NMR spectroscopy or ion-selective electrodes (LUVs have a diameter of 100-200 nm, SUVs (small unilamellar vesicles) 30-50 nm, and GUVs (giant unilamellar vesicles) >300nm G stands for giant and S for small). The use of LUVs is recommended for supramolecular chemists entering the field, because they do not require specialized equipment, provide a rapid overview on various activities, and... 

Figure 2. Left Schematic drawing of a unilamellar bilayer vesicle. Right Sketches of vesicles and liposomes of different size. The following abbreviations are commonly used MLV multilamellar vesicles, LUV large unilamellar vesicles, lUV intermediate unilamellar vesicles, SUV small unilamellar vesicles.

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