Vesicle giant unilamellar

GUVs Giant unilamellar vesicles >1 pm, huge trapped volume, unstable 9,10... 

Gudheti, M. V., Mlodzianoski, ML, and Ffess, S. T. (2007) Imaging and shape analysis of giant unilamellar vesicles (GUVs) as model plasma membranes Effect of fnms-DOPC (dielaidoyl phosphatidylcholine) on membrane properties. Biophys. J. 10.1529/biophysj.106.103374. 

Fig. 5 Membrane models for NMR structure analysis, (a) An isotropic detergent micelle (left) is compared to the dimensions of lipid bilayers (right), (b) Macroscopically oriented membrane samples can be prepared on solid support, as nanodiscs, or as magnetically oriented bicelles. (c) Nomenclature and variability of liposomes small (SUV, 20-40 nm), intermediate (IUV, 40-60 nm), large (LUV, 100-400 nm), and giant unilamellar vesicles (GUV, 1 pm) multi-lamellar (MLV), oligo-lamellar (OLV) and highly heterogeneous multi-oligo-lamellar vesicles (MOLV)...

Yamashita Y, Masum SM, Tanaka T, Tamba Y, Yamazaki M (2002) Shape changes of giant unilamellar vesicles of phosphatidylcholine induced by a de novo designed peptide interacting with their membrane interface. Langmuir 18 9638-9641. 

Gangl, Susanne, Molecular Organization on Giant Unilamellar Vesicles,... 

Manneville, Jan-Baptiste, Magnification of Shape Fluctuations of Active Giant Unilamellar Vesicles, 6, 351. 

Liposomes occur in nature, but can also be easily synthesized in the laboratory. Depending on the preparation method used, whioh influenoes their size — in relation to the number of bilayer shells — and physical properties, liposomes are olassified as small unilamellar vesicles (SUVs, 25-50 nm), large unilamellar vesioles (LUVs, 100 nm to 1 pm), giant unilamellar vesicles (GUVs, 1.0-200 pm) multilamellar vesioles (MLVs, 0.1-15 pm), and multi-vesicular vesicles (MWs, 1.6-10.5 pm) the last consists of several small vesicles. Bicelles, which contain surfactant molecules in the lipid bilayer, constitute a special type of liposome. 

Montes, L.R., Alonso, A., Goni, F.M., and Bagatolli, L.A. (2007) Giant unilamellar vesicles electroformed from native membranes and organic hpid mixtures under physiological conditions. Biophysical Journal, 93, 3548-3554. 

Pott, T., Bouvrais, H., and Meleard, P. (2008) Giant unilamellar vesicle formation under physiologically relevant conditions. Chemistry and Physics of Lipids, 154 (2), 115-119. 

Inaoka, Y. and Yamazaki, M. (2007) Vesicle fission of giant unilamellar vesicles of liquid-ordered-phase membranes induced by amphiphiles with a single long hydrocarbon chain. Langmuir, 23 (2), 720-728. 

L.A. Bagatolli and E. Gratton. Two-photon fluorescence microscopy of coexisting lipid domains in giant unilamellar vesicles of binary lipid mixtures. Biophys. 

S.A. Sanchez, L.A. Bagatolli, E. Gratton, and T. L. Hazlett. A two-photon view of an enzyme at work Crotalus atrox venom PLA2 interaction with single-lipid and mixed-lipid giant unilamellar vesicles. Biophys. J., 2002, 82, 2232-2243. 

Angelova, M.I. et al.. Shape transformations of giant unilamellar vesicles induced by ethanol and... 

Winter and co-workers elucidated the interaction between lipidated Ras protein and membrane and investigated the distributiOTi of Ras proteins in membrane microenvironments using two-photon fluorescence microscopy on giant unilamellar vesicles (GUVs) and tapping mode atomic force microscopy (AFM)... 

The A-T base pair formation at the vesicular surface was also shown to induce hemifusion of bdayer membranes of giant unilamellar vesicles (10-150 pm in diameter) which consisted of dioleylphosphatidylcholine containing deoxyadenosine (8) or deoxythimine (9)-functionalized lipids (5 w/w%), which gives some insight into the growing process of the assemblies [26]. 

An excellent compromise in simplicity and value of information is found in the use of LUVs, which can be probed by various complementary methods such as fluorescence and NMR spectroscopy or ion-selective electrodes (LUVs have a diameter of 100-200 nm, SUVs (small unilamellar vesicles) 30-50 nm, and GUVs (giant unilamellar vesicles) >300nm G stands for giant and S for small). The use of LUVs is recommended for supramolecular chemists entering the field, because they do not require specialized equipment, provide a rapid overview on various activities, and... 

Figure 1 Top Small unilamellar vesicles (SUVs), large unilamellar vesicles (LUVs), giant unilamellar vesicles (GUVs), and multilamellar vesicles (MLVs). Bottom Dynamic processes in bilayer membranes involve exchange (fcex), lateral diffusion (jfcdiff), and flip-flop (%).

Electroformation A method used to create giant unilamellar vesicles, in which an alternating current is used to desorb lipids from a conducting surface. 

Giant unilamellar vesicle (GUV) A vesicle with a single bilayer membrane and a diameter >1 pm. 

The hposome inside a hposome configuration is analogous to a vesicle inside a secretory cell during the last stages of the release process. Microelectroporation (77) assisted insertion and careful micromanipulation of a micropipette is required for formation of the artificial cell. Briefly, a micropipette filled with the redox molecule of choice (typically catechol) is positioned next to a surface immobilized giant unilamellar vesicle (GUV) with... 

C) the solvent displacement method, and (D) the double emulsion method for giant unilamellar vesicles. 

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