Suspensions, preparation

Polymerization of Lipid 1 by UV Irradiation. The vesicle suspension prepared as described above was transfered into a quartz tube which was then flushed with nitrogen gas for about 20 minutes. After the tube was sealed with a rubber stopper, it was put on a rotator contained in a miniphotoreactor for UV irradiation for 3 minutes with slow rotation. 

An example of the second method of parenteral suspension preparation is testosterone suspension. Here, the vehicle is prepared and sterile-filtered. The testosterone is dissolved separately in acetone and sterile-filtered. The testosterone-acetone solution is aseptically added to the sterile vehicle, causing the testosterone to crystallize. The resulting suspension is then diluted with sterile vehicle, mixed, the crystals allowed to settle, and the supernatant solution siphoned off. This procedure is repeated several times until all the acetone has been removed. The suspension is then brought to volume and filled in the normal manner. 

In-process controls, such as the checking of weights and disintegration or dissolution of tablets, satisfactory mixing, appropriate suspension preparation, or clarity of solutions must be conducted to ensure appropriate product content uniformity and performance... 

Solute uptake can also be evaluated in isolated cell suspensions, cell mono-layers, and enterocyte membrane vesicles. In these preparations, uptake is normalized by enzyme activity and/or protein concentration. While the isolation of cells in suspension preparations is an experimentally easy procedure, disruption of cell monolayers causes dedifferentiation and mucosal-to-serosal polarity is lost. While cell monolayers from culture have become a popular drug absorption screening tool, differences in drug metabolism and carrier-mediated absorption [70], export, and paracellular transport may be cell-type- and condition-depen-dent. 

The hot extraction method is a variation of this procedure. The aqueous pigment suspension, prepared as above, is refluxed for a certain time, usually 5 minutes, cooled rapidly, and filtered. A known amount of extract is then dried by evaporation and the weight of the residue determined, or the extracted pigment weighed and the dissolved portion determined by calculating the difference. 

Glende EA Jr, Lee PY. 1985. Isopropanol and chlordecone potentiation of carbon tetrachloride liver injury Retention of potentiating action in hepatocyte suspensions prepared from rats given isopropanol or chlordecone. Exp Mol Pathol 42(2) 167-174... 

Using the C60 suspension prepared from non-homogenized fullerene, the efficacy of inactivation of the vims was much lower compared to the fine suspension. The viral titer in this case decreased by one to two times, suggesting that the efficacy of inhibition of the vims strongly depends on the total surface of the fullerene exposed to oxygen and liquid. 

Batch equilibrium tests are conducted on solid phase suspensions, prepared with previously air-dried solids, ground to uniform powdery texture for mixing with various concentrations of the pollutants of interest in solution. The concentrations of these pollutants or the COMs leachate in the solution are designed to evaluate the capability of the suspended solids to adsorb all the pollutants possible with increasing amounts of available pollutants, consistent with interaction characteristics dictated by the surface properties of the solids and the pollutants [1,16,22-26,66,67,71]. For a successful and proper study of solid particle sorption of pollutants, the requirement for complete dispersion of solid particles in solution is absolute [143 -145]. Common practice is to use a solution to solid ratio of 10 1 [1], together with efficient sample agitation at a constant temperature (e.g.,48 h at 20 °C). 

A colloidal suspension prepared according to the method described in Section 13.2.3 was contacted with a porous alumina carrier to obtain a bimetallic palladium-tin catalyst. Evaluation of the catalytic properties of this system is detailed... 

To a Candida udlis suspension, prepared as described above, individual l,3-/ -glucanases were added at either a lower level of 41 units/ml or an upper level of 102.5 units/ml to the reaction mixtures according to the following planning experiment matrix (equation 3). Each row of the matrix represents an elementary experiment of which result y is contained in the result s matrix Y. 

We found [2] that a number of ketone carbinols can be prepared in excellent yields by adding the ketone to the suspension, prepared by introducing an excess of acetylene into a solution of 1-BuOK in THF. This method shows some resemblance with the Favorski preparation. The reactive intermediate is probably potassium acetylide or a complex of it with r-BuOH or acetylene. 

Agitation levels 7-10 are characteristic of applications requiring high fluid velocities for process result, such as mixing of the high viscosity suspension preparations. 

Suspensions prepared from alginic acid, obtained from Magrocystis pyrlfera (kelp)... 

Fig. 15.1. LCM concentrations during Filmix suspension preparation measured using optical particle counters. (Taken from ref. 717. See text for further discussion.)...

Barnes, H.A. Holbrook, S.A. High Concentration Suspensions Preparation and Properties In Processing of Solid-Liquid Suspensions, Shamlou, P.A. (Ed.), Butterworth-Heine-mann Boston, 1993, pp. 222-245. 

Bone marrow is placed into a balanced salt solution containing preservative-free heparin and a single cell suspension prepared by passing the marrow through a fine wire mesh. Alternatively, a small Potter-Elvehjem tissue homogenizer (Hll) may be used. The nucleated cell count is adjusted to 5-10 X 106 ml of balanced salt solution and deoxyuridine added. Following incubation, 5 p,Ci of tritiated thymidine is added and the mixture incubated for an additional hour. The cells are then washed and the nucleated cell count determined. A 0.1 ml aliquot is then placed on a filter paper disk and dried. The activity of the dried disks is then measured in a scintillation... 

A suspension prepared from 4.25 g. of 95% sodium borohydride (0.11 mole) and 150 ml. of tetrahydrofuran is cooled in an ice-water bath and stirred while a solution containing 17 ml. (0.13 mole) of boron trifluoride-diethylether in 60 ml. of tetrahydrofuran is added dropwise over a period of 45 minutes. Thirty grams (0.11 mole) of triphenylphosphine is dissolved in 130 ml. of tetrahydrofuran, and this solution is added through a dropping funnel to the cooled suspension (1 hour). The mixture is allowed to warm to room temperature. Solids are collected by filtration, and the crude product is recovered by evaporation of the solvent from the filtrate in vacuo. Yield is 32 g. (100% theory). 

Though the majority of this chapter is related to the production of tubular supports, a few comments on the preparation of flat supports will be made. Emphasis is put on the stabilisation of the suspensions used in the preparation of the supports. Experimental procedures are provided in more detail in chapter 4 of this thesis the present chapter mainly provides the basic knowledge for suspension preparation and shaping techniques. 

In summary, we have the following steps (1) cell suspension, preparation, viability, and density (2) cells in agarose gel on slides (3) lysis (4) DNA unwinding, electrophoresis, neutralization, and staining with a fluorophore (5) image analysis and scoring. 

Figure 4.1 Gold loading and yield of DP versus solution pH for Au/Ti02 (Degussa P-25) catalysts prepared by DP (NaOH added to the suspension, preparation at 343K, nominal Au loading 13wt.%).62...

Approximately 24,48, or 72 hours after dosing, mice killed and bone marrow obtained from both femurs Slide of a bone marrow suspension prepared, stained with Wright-Giemsa stain, dried, and scored blind... 

There is a difference in bioequivalence between capsules and phenytoin suspension. A 100-mg phenytoin capsule is equivalent to 90 mg of the suspension preparation. 

The persistent foam at the top of 100ml of suspension prepared in WHO standard hard water (WHO method WHO/M/29 see Annex 2, section II) with 5ml of emulsion, oil in water, shall not exceed 10ml when tested by CIPAC method MT 47 (2). 

The enzyme activity was obtained using a microsomal suspension prepared from mouse liver. 

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