Sulfosuccinimidyl-4- cyclohexane-1 -carboxylate

Sulfosuccinimidyl 4-(N-maleimidomethyl)-cyclohexane-1 -carboxylate Water Soluble MW 436.37... 

Sulfo-SMCC sulfosuccinimidyl 4-N-maleimidomethyl cyclohexane-1-carboxylate... 

Fig. 12 Illustration of the chemistry employed for preparation of a modified Si(lll) surface. Firstly, a layer of alkenyl acid is bound to the surface by covalent attachment. Secondly, a layer of polylysine is electrostatically bound to the carboxylic activated surface and finally the DNA is bound to the surface in a reaction mediated by sulfosuccinimidyl 4-(N-maleimidomethyl)-cyclohexane-l-carboxylate [27]...

Therefore, surface modification strategies for the formation of direct silicon-carbon bonds require, first, a special pre-treatment of the silicon surface to prevent oxidation and, second, an activation of the silicon surface for subsequent reaction with organic moieties. This has been achieved by treatment of the silicon surface with hydrofluoric acid to generate a hydrogen-terminated Si(lll) surface, which can further react with unsaturated co-functionahzed alkenes in the presence of UV irradiation or by thermal activation [27,44,45]. Using this method, carboxylic acid modified silicon substrates have been successfully generated and coupled to thiol modified ONDs via a polylysine/sulfosuccinimidyl 4-(M-maleimidomethyl)-cyclohexane-l-carboxylate couphng (Fig. 12). 

Heterobifunctional cross-linkers are employed to couple two different functional groups between the monolayer and the molecule for the subsequent immobilization. The wide range of these cross-linkers and their specific reactions are presented in Figure 14.7. One example in which the use of heterobifunctional cross-linkers was applied for immobilization of biomolecules was the work of Com and coworkers where the sulfosuccinimidyl 4-(fV-maleimidomethyl)cyclohexane-l-carboxylate (SSMCC) linker was applied to link thiol-modified DNA molecules with an amino-functionalized surface.54... 

Centricon 30 filtration units (Amicon) or Filtron Microsep 30 (Filtron) PDIO gel filtration medium (Pharmacia) 5(6)-carboxyfluoroscein-N-hydroxysuccinimide ester Boehringer cat. 10055089 stock solution, 10 mg/ml in DMSO Sulfo SMCC [sulfosuccinimidyl 4-(/V-maleimidomethyl)cyclohexane-l-carboxylate]. Pierce Chemical Company (cat. 22322), 20 mg/ml stock solution in 50 mM borate buffer, pH 7.6, freshly prepared... 

PM, polyacrylamide y- PS, y-amlnopropylsiloxane DMSA, weso-2,3-dimercaptosuccinic acid SMCC, sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1 -carboxylate HA, hydroxyapatite CB, carborane Na oL, sodium oleate CP, cisplatin DR, doxorubicin PEG, polyethylene glycol. 

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