Stress proteins effect

Low-Friedrich, I. Schoeppe, W. (1991). Effects of calcium channel blockers on stress protein synthesis in cardiac myocytes. J. Cardiovasc. Pharmacol. 17,800-806. 

Most of the stress proteins discussed in this account have a role either in helping the plants survive, or in minimising the effectiveness of the stress agent. In helping plants survive under stress conditions, the stress proteins perform the following functions (1) maintenance of the basic metabolism in the stressed cell, e.g. the induction of ADH and some... 

Dunlop [18] proposed a model for sub-lytic effects in plant cells, based on the same principles, but including four properties postulated to be of particular importance in these systems, namely calcium ion flux, osmo-regulation, cell-cell contact/aggregation and stress protein expression. Of these factors, osmo-regulation (and its inter-relationship with the cell wall) and aggregation patterns, in particular, distinguish plant cells from mammalian cell systems. 

There is a perceived need to develop effective vaccines against bovine spongiform encephalopathy (BSE) and the human form, new variant Creutzfeldt-Jacob (nvCJ) disease. Organ transplant vaccines would obviously be beneficial and tetanus toxoid has been shown to lower cholesterol in animals. Tumor-specific antigens have also been explored and heat stress proteins have been evaluated as adjuvants in this case. 

A dipeptide Met- , derived from sardine muscle (Matsufuji et ah, 1994), stimulates expression of the antioxidant defense protein HO-1 in a concentration-dependent manner. Previous findings revealed that HO-1 protein expression is accompanied by the induction of a secondary antioxidant protein, ferritin. In a present study, the effect of Met- on the expression of the antioxidant stress proteins, heme oxygenase-1 (HO-1), and ferritin in endothelial cells derived from the human umbilical vein and their contribution to the decrease in radical formation that occurs under the influence of this dipeptide were studied and reported potential activity (Erdmann et ah, 2006). 

Traditionally, the production of mAbs uses complex culture media containing glucose and amino acids as the main sources of carbon for cell metabolism, as well as vitamins, micronutrients and sometimes animal serum, usually fetal bovine serum. Chapter 5 provides a discussion on composition of culture media and recent trends in the search for formulas that do not require the use of animal serum, or of proteins of animal origin. These serum-free formulations use substitutes such as peptones, epithelial and fibroblast growth factors, hydrolysates, yeast extract, choline, and inositol. For the production of mAbs, various serum-free formulas are available, some of these developed specifically for a given cell line (Chu and Robinson, 2001). The development of those media is easier for non-anchorage-dependent cells, such as those used for mAb production. Thus, approximately 50% of the antibodies for therapeutic use are already produced using serum-free media. In some circumstances, the elimination of serum should be accompanied by the addition of other substances with the same shear stress protective effect of serum proteins,... 

Goldbaum O, Richter-Landsberg C (2001) Stress proteins in oligodendrocytes differential effects of heat shock and oxidative stress. J Neurochem 78 1233-1234... 

Members of the hspVO family of stress proteins bind the GSL 3 sulfogalactosyl ceramide (SGC) (207). The binding site is in the N-terminal ATPase domain (208). Adamantyl SGC, similarly generated via fatty acid replacement, similarly has proven water soluble and is an effective inhibitor of hsp70-SGC binding (200). Adamantyl SGC has been shown to inhibit hsp70 ATPase activity (209) in vitro and therefore may modulate its chaperone function in cells. Such an effect also has therapeutic potential (210). 

Recently a move towards the assessment of cellular damage, chemical effects on enzyme or stress proteins and the relationship towards environmental assessments of contaminants have become more accepted within the regulatory... 

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