Streptomycin sulphate

Seek T75 plastic tissue culture flasks with a minimum of 2.5 x 106 cells in 120ml of Eagle s medium containing 20mM L-glutamine 0.88g l-1 sodium bicarbonate 20 mM HEPES 50 pg ml-1 streptomycin sulphate 50IUml 1 benzyl-penicillin and 7.5% fetal bovine serum. The flasks are incubated for 18-24 h at 37°C in a C02 incubator to establish monolayer cultures. 

Storage. Gravid proglottides should be stored in normal saline containing antibiotics, penicillin G (100 i.u./ml), streptomycin sulphate (100 / g/ml) and myostatin (100 fig/ml), as soon as possible after being passed. 

Boil a small quantity of streptomycin sulphate with M sodium hydroxide, add a slight excess of hydrochloric acid and a few drops offerric chloride solution—violet. 

Streptomvces nodosus, 350 Streptomyces noursei, 829 Streptomyces orchidaceus, 504 Streptomyces onentalis, 1060 Streptomyces peuceticus, 575 Streptomycin, 976 Streptomycin sulphate, 976 Streptosil, 991... 

With the differential pulse polarography [245], the antibiotics can be determined at low concentration, if necessary, at the ppm or even sub-ppm level. Tetracycline hydrochloride is determined in aqueous acetate buffer pH 4 (detection limit 0.1 ppm), but for the analysis of chlortetracycline hydrochloride, oxytetracycline hydrochloride and free tetracycline, a non-aqueous medium must be used. Streptomycin sulphate is analysed in alkaline solution, trace quantities of zinc being masked by Na2EDTA, and the detection limit is 1 ppm. A determination in blood serum or urine is also possible but the peak potentials are shifted here to more negative values. The polarographic determination is preceded by ultrafiltration. Penicillin G potassium and ampicillin must be first functionalised by nitrosation. The authors also recommend an analysis of mixtures which is however demonstrated only with chloramphenicol and tetracycline, at 2.4 and 4.2 ppm, respectively. 

Cells were suspended in lysis buffer, 50 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 0.2 M benzamidine-HCl, 0.2 mM PMS, pH 7.5 and lysed using ultrasonication. Streptomycin sulphate (1%) and polyethyleneimine (0.1%) were added to precipitate DNA. The extract was clarified by centrifugation. 

A. Strepton dn INN, Streptomycin Sulphate BAN, Streptony/cin Sulfate USAN,... 

Rasmussen F (1972) Side effects of streptomycin sulphate. Scand J Respir Dis 53 35-37... 

The solubility of dihydrostreptomycin sulphate, which is water soluble, is decreased in concentrated solutions of sorbitan monolaurate (E = 12) [129]. Streptomycin sulphate is also very soluble in water and is incompatible with sodium lauryl sulphate. Combination of streptomycin with polysorbate 20 produces a strong bacteriostatic effect against antibiotic resistant bacteria. Intrapleural injection of 0.5 g dihydrostreptomycin with one drop of polysorbate 20 in 4 ml has been reported to result in a sterile pleural sample 1 week after injection [130]. 

Natori [178] found that the addition of 0.05% polysorbate 80 decreased the activity of isoniazid, 4,4 -diaminodiphenylsulphone, oleic acid, and 3-aminoben-zofuran, but had no significant effect on streptomycin sulphate. The activity of isoniazid and 4,4 -diaminodiphenylsulphone was, however, reported to be increased by the addition of surfactants under different conditions [176]. 

Pipette a volume of sample containing 2 5 g (2,500,000 units) of streptomycin sulphate into a 100-ml graduated fiask and dilute to volume with water. Dilute a 5-ml aliquot of this solution to 500 ml with water in a graduated flask, then transfer a 5-ml aliquot of this final dilution to a test-tube and add exactly 1 ml of 2N sodium hydroxide. Mix and place the tube in a water-bath. Exactly three minutes after the addition of the alkali, remove the tube from the bath and cool under a cold-water tap for exactly three minutes. At the end of this time add, by pipette, 4 ml of a 1 per cent solution of ferric ammonium sulphate in 0 75 N sulphuric acid, shake and allow to stand. After exactly ten minutes, measure the extinction at 550 mju, using 2-cm cells with water in the comparison cell. Read the concentration of streptomycin in the final 5-ml aliquot prepared from the sample from a standard curve. 

The standard curve is prepared as follows Transfer suitable volumes, covering the range 0 to 2 0 mg of a standard solution of streptomycin sulphate containing 0 5 mg per ml, to a series of test-tubes and dilute each to 5 ml with water. Treat the contents of each tube exactly as described above from add exactly 1 ml of 2N sodium hydroxide. . . and ending with .. . water in the comparison cell. Prepare a curve by plotting streptomycin sulphate content against extinction. 

---