Chlortetracycline hydrochloride

Chlortetracycline hydrochloride (CTC-HCL) is the hydrochloride salt of an antibiotic substance produced by the growth of Streptomyces aureofaciens (Fam. Streptomycetaceae). 

The current official FDA Chlortetracycline Working Standard is chlortetracycline hydrochloride, Lot //501-632B-95-1 (9/29/53), obtained from Lederle, which markets the antibiotic under the proprietary name Aureomycin. The current working standard has an assigned potency of 1000 yg/mg (the term yg applied to chlortetracycline means the chlortetracycline activity (potency) contained in 1 yg of the FDA Chlortetracycline Master Standard (Lot 990-107-141-1), which is also chlortetracycline hydrochloride). 

W. Najdong, J. De Beer, X. Marcelis, P. Derese, J. H. M. C. B. Miller, and J. Hoogmartens, Collaborative study of the analysis of chlortetracycline hydrochloride by liquid chromatography on polystryrene divinyl benzene packing materials, J. Pharm. Bio-med. Anal., 10 199 (1992). 

Infra-red Spectrum. Principal peaks at wavenumbers 1622,1580, 1666, 1311, 1041, 1227 (chlortetracycline hydrochloride, KBr disk). Polymorphism may occur. 

Chlortalidone, 464 Chlortet, 463 Chlortetracycline, 463 Chlortetracycline calcium, 463 Chlortetracycline hydrochloride, 463 Chlorthal dimethyl, 82 Chlorthalidone, 464 Chlorthiamid, 82 Chlortoluron, 82 Chlor-Trimeton, 457 Chlortripelennamine, 648 Chlor-Tripolon, 457 Chlortritylimidazol, 487 Chlorzide, 663 Chlorzoxazone, 465 Chlosudimeprimyl, 483 Chlotride, 454 Cholecalciferol, 465 Choledyl, 1011 Cholestenol, 466 Cholesterin, 466 Cholesterol, 466 Choline, 466 (metabolite), 999 Choline acid tartrate, 466 Choline bitartrate, 466... 

With the differential pulse polarography [245], the antibiotics can be determined at low concentration, if necessary, at the ppm or even sub-ppm level. Tetracycline hydrochloride is determined in aqueous acetate buffer pH 4 (detection limit 0.1 ppm), but for the analysis of chlortetracycline hydrochloride, oxytetracycline hydrochloride and free tetracycline, a non-aqueous medium must be used. Streptomycin sulphate is analysed in alkaline solution, trace quantities of zinc being masked by Na2EDTA, and the detection limit is 1 ppm. A determination in blood serum or urine is also possible but the peak potentials are shifted here to more negative values. The polarographic determination is preceded by ultrafiltration. Penicillin G potassium and ampicillin must be first functionalised by nitrosation. The authors also recommend an analysis of mixtures which is however demonstrated only with chloramphenicol and tetracycline, at 2.4 and 4.2 ppm, respectively. 

Aureomycin (chlortetracycline hydrochloride) Sugars Streptomyces aureofaciens... 

Chlortetracycline Hydrochloride. USP. Chlonctracy-dine (Aureomycin hydrochloride) was isolated by Dug-gar"" in 194S from S. uiireofaciens. This compound, whith was produced in an cxtcn.sivc. search fur new antibiotics, was the first of the group of highly successful tetracyclines. It soon became established as a valuable antibiotic with broad-spectrum activities. 

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