Streptomyces faecalis

When the resulting mixture of benzoylformic acid and (i )-mandelic acid was treated with a cell free extract of Streptomyces faecalis IFO 12964 in the presence of NADH,the keto acid can be effectively reduced to (i )-mandelic acid (Fig. 1). Fortunately the presence of A. bronchisepticus and its metabolite had no influence on the reduction of the keto acid. The regeneration of NADH was nicely achieved by coupling the reaction with reduction by formic acid with the aid of formate dehydrogenase. As a whole, the total conversion of racemic mandelic acid to the i -enantiomer proceeded with very high chemical and optical yields. The method is very simple and can be performed in a one-pot procedure [6]. 

The poly(HA) depolymerases of the bacteria Alcaligenes faecalis (strains AE122 and Tl), Comamonas acidovorans, Comamonas testosteroni, Comamonas sp., Pseudomonas fluorescens, Pseudomonas lemoignei, Pseudomonas stutzeri, Ralstonia pickettii, Streptomyces exfoliatus, and of the fungi Paecilomyces lilaci-nus, Penicillium funiculosum, and Penicillium pinophilum have been purified and characterized (for details see Table 1). Poly(HA) depolymerases share several characteristics ... 

Staphylococcus aureus, Streptomyces pyogenes, and Enterococcus faecalis. In 2002, Qian-Cutrone and coworkers isolated stephacidins A (163) and B (164) from A. ochraceus WC76466 [115]. Stephacidins A... 

The azirinomycin (12), 3-methyl-2H-azirine-2-carboxylic acid, was isolated from a strain of Streptomyces aureus. Its methyl ester (13) exhibited broad spectrum antibiotic activity in vitro against both Gram-positive and Gram-negative bacteria [33, 34]. The carboxylic acid (12) is most active against Staphylococcus aureus followed by Proteus vulgaris. Bacillus subtilis, and Streptococcus faecalis. In contrast, the methyl ester shows its lowest activities against one of the Staphylococcus aureus cultures and Streptococcus faecalis. 

Data from labelling experiments with the disaccharide-peptide monomers and peptide-cross-linked dimers and trimers found in the peptidoglycan of Streptococcus faecalis have shown that the peptide chains of peptidoglycans are cross-linked into dimers and trimers, etc., by a monomer-addition mechanism rather than by random cross-linking. Similarly, the in vitro synthesis of a peptide trimer by the extracellular transpeptidase of Streptomyces R61 is not a random process. Synthesis occurs preferentially by transpeptidation between a peptide monomer, acting as a donor, and a preformed peptide dimer, acting as an acceptor. 

Biphenomycin B 11 is a 15-membered cyclic tripeptide isolated from the culture broths of Streptomyces filipinensis and S. griseorubuginosus. This compound displayed potent activity against Gram-positive, p-lactam-resistant bacteria, such as Streptococcus aureus. Enterococcus faecalis, or Streptococcus sp. The enantioselective total syntheses of biphenomycin B 11 using an asymmetric phase-transfer alkylation were reported by two groups. 

ValDH NAD, NADP Bacteria Streptomyces, Alcaligenes faecalis, Planococcus), plants (pea, wheat)... 

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