Stratus analyzer

The system is fitted with a sample preparation unit (Fig. 51) (the use of which depends on the methods to be employed). The analysis apparatus is 

The method is based on the principle of enzyme immunoassay with fluorimet-ric detection and is termed radial partition immunoassay. This test can analyse macromolecules and haptens rapidly and with high sensitivity in a fully mechanised analysis system. The special feature of this test is the use of carrier-bound reagents on a so-called dry tab. 

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ST26 Verstraete. A.G. and Wieme, R.J. (1986). An enzymatic factor may interfere with the digoxin on the Stratus analyzer. Ther. Drug. Monit. 8, 244. 

ST30 Rugg, J.A., Flaa, C.W., Dawson, S.R., Rigl, C.T., Leung, K.S., Knight, W.R. and Evans, S.A. (1987). Radial partition immunoassay Rapid automated quantification of human thyrotropin on the Stratus analyzer. Clin. Chem. 33, 882, Abstr. 11. 

ST35 Armbruster, D. and Jirinzu, D.C. (1988). Empty sample cups may mimic patients results with the Stratus analyzer. Clin. Chem. 34, 1931. 

ST63 Mahmood, N., Srebro, S., McCarthy, M., Brotherton, M., Hunter, T. and DeMarco, C. (1989). Rapid automated determination of total prolactin on the Stratus analyzer. Clin. Chem. 35, 1095, Abstr. 141. 

ST79 Chapelle, J.-P. and El Allaf, M. (1990). Automated quantification of creatine kinase MB isoenzyme in serum by radial partition immunoassay, with use of the Stratus analyzer. Clin. Chem. 36, 99-101. 

Commercial application of the dendrimer-based reagent technology has been demonstrated by the successful development of The Stratus CS STAT fluorometric analyzer [5] marketed by Dade Behring Inc. This rapid automated point of care immunoassay system provides quantitative analysis of whole blood or preprocessed plasma samples via unit use assay test packs. Up to four test packs can be introduced for each sample. All reagents [5-9] required for specimen analyses are contained within the test packs. 

As described above, the dendrimer-coupled antibody conjugates show uniquely enhanced properties compared to the classical double antibody systems. Important characteristics such as complete solubility in aqueous buffers, flexibility in immunoassay format, ability to improve assay sensitivity, consistent, reproducible manufacturing and favorable stability has driven the utilization of these dendrimer-based reagents in Stratus CS, the latest member of the Stratus family of immunochemistry analyzers. In this new analyzer system, the primary... 

In the new Stratus CS system results are available in less than 15 min after sample draw and the system has the capability to analyze four samples in less than 30 min. Ease of use, analytical sensitivity, accuracy, precision, and reproducibility makes this system suitable for use in chest pain centers, emergency departments, critical care units, observation wards and clinical laboratories. 

Cronin, P., Evers, T., Mozza, J., Bauer, R., Hickey, G. and Kamm, C. A flexible system for the quantitative analysis of cardiac markers with minimized total turnaround time the Stratus CS Analyzer , Clin. Chem., 44, 542 (Abstract) (1998). 

Stengelin, M., Hudson, K., Bedzyk, W. D., Singh, P., Desai, V., LeBlanc, M., Berg, M., Murray, V. and Bauer, R. Development and performance of a high-sensitivity C-Reactive Protein assay for the Stratus CS STAT fluorometric analyzer , Clin. Chem., 46, 26 (Abstract) (2000). 

Henkel, E. (1985). Evaluation of the Stratus radial partition immunoassay analyzer - Comparison with HPLC and RIA. Clin. Chem. 31,922, Abstr. 100. Henkel, E. and Arslan, E. (1985). Erfahrungen mit dem Stratus-Immunoassay-System. GIT Lab.-Med. 8, 218-224. 

ST71 Ryan, F., Scott, D., Gregory, M. and Turner, M. (1989). Measurement of creatine kinase MB by the Baxter Stratus fluorometric analyzer. Clin. Chem. 35, 1123, Abstr. 271. 

Analyzer Ektachem 700 Ektachem DT-60 Reflotron Cobas Ready, Spotchem Seralyzer Stratus Opus... 

Kamm C, Elser R, Eitel D, Napier J. Evaluation of the Stratus CS analyzer and three cardiac markers. Clin Chem 1998 44 1457-63. 

Chemiluminescent and fluorescent labels provide increased sensitivity with less background, for a much higher signal-to-noise ratio. Some examples of automated analyzers are Abbott IMx and Commander , Baxter Stratus , Kodak Amerlite , and Hybritech Photon QA . With the increased sensitivity and expanded menu for drug monitoring, researchers in pharmaceutical bioanalytical laboratories should be able to utilize such automation, provided that cautions in Secs. IV.B are taken into consideration. 

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