Radial partition immunoassay

Antibodies have been coupled1271 to starburst (PAMAM) dendrimers these reagents offer an attractive approach to the development of immunoassays. These dendritic reagents are stable and retain full immunological activity - both in solution as well as when bound to a solid substrate their analytical sensitivity is equal to or is better than that of the established methods. The dendritic approach to radial partition immunoassays has been shown to possess the best traits of both homogeneous and heterogeneous immunoassay formats. 

Recent developments show that dry chemistry has also been advancing in the sector of immunological detection methods. The homogeneous immunoassay technique facilitated access to the immunological detection method. The substrate-bound fluoro-immunoassay (SLFIA) serves as an example for the determination of theophylline concentration. However, the radial partition immunoassay paved the way for determining the concentration of numerous drugs and hormones. 

The method is based on the principle of enzyme immunoassay with fluorimet-ric detection and is termed radial partition immunoassay. This test can analyse macromolecules and haptens rapidly and with high sensitivity in a fully mechanised analysis system. The special feature of this test is the use of carrier-bound reagents on a so-called dry tab. 

Henkel, E. (1985). Evaluation of the Stratus radial partition immunoassay analyzer - Comparison with HPLC and RIA. Clin. Chem. 31,922, Abstr. 100. Henkel, E. and Arslan, E. (1985). Erfahrungen mit dem Stratus-Immunoassay-System. GIT Lab.-Med. 8, 218-224. 

ST14 Sheiman, M., Srebro, S., Tokarski, R., Knight, W. and Giegel, J. (1985). Radial partition immunoassay Monitoring amikacin levels in serum und plasma. Clin. Chem. 31, 933, Abstr. 160. 

ST16 Cilissen, J., Hooymans, P.M. and Merkus, F.W.H.M. (1986). Modified fluorescence polarization immunoassay compared with radial partition immunoassay and a radioimmunoassay for digoxin. Clin. Chem. 32, 2100. 

ST17 Goodnow, T., Timmons, R., Koski, K., Soto, A., Kennedy, S. and Evans, S. (1986). Radial partition immunoassay Monitoring ferritin levels in serum and plasma. Clin. Chem. 32,1143, Abstr. 461. 

ST30 Rugg, J.A., Flaa, C.W., Dawson, S.R., Rigl, C.T., Leung, K.S., Knight, W.R. and Evans, S.A. (1987). Radial partition immunoassay Rapid automated quantification of human thyrotropin on the Stratus analyzer. Clin. Chem. 33, 882, Abstr. 11. 

Radial partition immunoassay A rapid, automated determination of serum or plasma vancomycin. Clin. Chem. 34, 1257, Abstr. 511. 

ST42 Plaut, D. and Johnson, C. (1988). Radial partition immunoassay A description and evaluation. J. Clin. Immunoassay 11, 81-85. 

ST65 Patel, N., Oppermann, M. and Bowman, C. (1989). Evaluation of radial partition immunoassay for the quantitative determination of immunoglobulin E. Clin. Chem. 35, 1199, Abstr. 632. 

ST79 Chapelle, J.-P. and El Allaf, M. (1990). Automated quantification of creatine kinase MB isoenzyme in serum by radial partition immunoassay, with use of the Stratus analyzer. Clin. Chem. 36, 99-101. 

ST86 Goodnow, T., Koski, K., Knez, R., LeBlanc, M., Bauer, R., Brotherton, M., Hoglen, J., Hunter, T., Waters, J., Gamble, J. and Houle, P. (1990). A radial partition immunoassay for the quantitation of alpha-fetoprotein. Clin. Chem. 36, 1088, Abstr. 638. 

ST98 Mifflin, T.E., Roberts, R.B., Barlow, E.H., Kocher, C.A., Schnatterly, P. and Felder, R.A. (1990). Maternal serum alphafetoprotein measured by radial partition immunoassay on the Stratus immunoassay system. Clin. Chem. 36, 1088, Abstr. 637. 

STN7 Erdmann, M.D. and Holt, J.A. (1991). Medians for intact human chorionic gonadotropin during second trimester of pregnancy (14 thru 20 wks) using automated radial partition immunoassay. Clin. Chem. 37, 940, Abstr. 146. 

STN18 Regan, J., Mahmood, N., Alvarez, W., Gamble, J., Sissors, D., Knez, R., Delgado, J. and Rugg, J. (1991). Automated quantification of estradiol by radial partition immunoassay. Clin. Chem. 37,936, Abstr. 128. 

Giegel JL, Brotherton MM, Cronin P, et al. Radial partition immunoassay. Clin Chem 1982 28 ... 

Leung K, Dawson S, Rugg J. A rapid automated radial partition immunoassay for the measurement of human lutropin. Clin Chem 1988 34 1221. 

Castillo M, Linne A, Soto A. A radial partition immunoassay for cortisol. Clin Chem 1984 30 1042. 

Morejon C, Soto A, Timmons P, Delgado J, Singh P, Ni WC, et al. Radial partition immunoassay for triiodothyronine. Clin Chem 1988 34 1213. 

---