Staphylococcal enterotoxin antibodies

Direct detection of Staphylococcal enterotoxins B (SEB) is illustrated in Fig. 14 which shows binding of SEB to the wavelength-modulated SPR sensor surface coated with respective antibodies for five different SEB concentrations". Figure 15 shows the sensor response to binding after 30-minute SEB incubation and initial binding rate as a function of SEB... 

In one report, an array of antigens patterned on a substrate was constructed for detection of IgG antibodies from various species. This method, termed miniaturized mosaic immunoassay, is rendered possible by using the PDMS channel for patterning materials [1027]. In another report, various antibodies were patterned via the PDMS channel on a substrate for detection of three antigens FI antigen (from Yersinia pestis), staphylococcal enterotoxin B, and D-dimer (DDi, a marker of sepsis and thrombotic disorder) [1028],... 

Detection of staphylococcal enterotoxin B (SEB), a causative agent of food poisoning, was achieved by QDs conjugated with polyclonal sheep anti-SEB antibody.57 Moreover, this approach also harbors the possibility of a multiplexed immunoassay (see Fig. 12.3), which was first reported by Goldman et al.58 in 2004 four toxins of interest in food- or water-borne illnesses (cholera toxin, ricin,... 

Thompson NE, Pattee PA (1977) Transformation in Staphylococcus aureus role of bacteriophage and incidence of competence among strains. J Bacteriol 129 778-788 Thompson NE, Razdan M, Kuntsmann G, Aschenbach JM, Evenson ML, BergdoU MS (1986) Detection of staphylococcal enterotoxins by enzyme-Unked immunosorbent assays and radioimmunoassays comparison of monoclonal and polyclonal antibody systems. Appl Environ Microbiol 51 885-890... 

Surgalla MJ, Bergdoll MS, Drack GM Use of antigen-antibody reactions in agar to follow the progress of fractionation of antigenic mixtures application to purification of staphylococcal enterotoxin. J Immunol 1952 69 357-365. 

Schroder E, Kunstmann G, Hasbach H, Pulverer G Prevalence of serum antibodies to toxic-shock-syndrome-toxin-1 and to staphylococcal enterotoxins A, B and C in West-Germany. Zentralbl Bakteriol Mikrobiol Hyg [A] 1988 270 110-114. 

Mrabet-Dahbi S, Breuer K, Klotz M, et al Deficiency in immunoglobulin G2 antibodies against staphylococcal enterotoxin ( defines a subgroup of patients with atopic dermatitis. Clin Exp Allergy 2005 35 274-281. 

Nedelkov et al. demonstrated the detection of staphylococcal enterotoxin B (SEB) in 2000 [37]. Antibodies to SEB were immobihzed on the sensing surface using EDC/NHS chemistry. This study demonstrated direct detection of concentrations ranging from 1 ng/mL to 100 ng/mL. Detections from 1 ng/mL to 100 ng/mL were also demonstrated in a milk matrix, as well as in a mushroom extract solution. In 2002, Homola et al. demonstrated improved detection Emits of SEB [38] (see Fig. 4). Using antibody immobilized via EDC/NHS chemistry, detections ranging from 0.5 ng/mL to 50 ng/mL were demonstrated. Detections were performed directly and using amplification with a secondary antibody. Detections for concentrations as low as... 

Antibodies to Staphylococcal enterotoxin A and E Enterotoxin A or E CNBr-activated Sepharose 4B 176... 

The staphylococcal enterotoxins are moderately stable proteins therefore, immunological evaluation should be possible on samples collected in either deployed or fixed medical treatment facilities. Immunoassays can detect picogram quantities of toxins in environmental samples. For comparison, 440 pg/mL was reported as the mean concentration of TSST-1 in human sera from patients with toxic shock syndrome.25 Anti-TSST-1 antibody titers are either suppressed or depleted in patients with toxic shock syndrome26,27 and the levels only recover during convalescence. In addition, most normal human serum samples contain detectable levels of antibody reacting with several different bacterial pyrogenic toxins, including... 

Lapeyre, C. Janin, F. Kaveri, S. V. Indirect double sandwich ELISA using monoclonal antibodies for detection of staphylococcal enterotoxins A, B, Cl, and D in food samples. Food Microbiol., 5 25-31. 1988. 

Figure 1.19. Plasma anti-toxin antibody levels of the IgM, IgA and IgG isotypes induced following oral immunizations with 100 pg doses of staphylococcal enterotoxin B vaccine either encapsulated (a) or free (b) (data from ref. (256))...

Eldridge, J. H., Staas, J. K., Meulbroek, J. A., Tice, T. R. and Gilley, R. M., Biodegradable and biocompatible poly(DL-lactide-co-glycolide) microspheres as an adjuvant for staphylococcal enterotoxin B toxoid which enhances the level of toxin-neutralizing antibodies. Infect. Immun., 59, 2978-2986 (1991). 

Delehanty and Ligler [5] used an antibody microarray system with continuous fluid flow to detect microbial toxins and simultaneously detected both cholera toxin and staphylococcal enterotoxin at a detection level as low as 8 and 4 ng/ml, respectively, within 15 min. Yang et al. [6] developed a device for detecting and genotyping influenza A viruses. Construction of the device utilizes solid-state electronics microfabiicated in silicon and glass, deposition of resistive heaters for thermal... 

Staphylococcal enterotoxin B (SEB) is a potent bacterial protein toxin responsible for food poisoning, as well as a potential biological warfare agent. An afBnity probe has been prepared by inunobilizing anti-SEB antibody on the surface of paratol-uene-sulfonyl-functionalized magnetic beads. Immobilization and affinity capture procedures were optimized to maximize the density of anti-SEB IgG on the surface of magnetic beads and the amount of captured SEB. MALDI-MS detection of the emiched SEB from different matrices, such as cultivation media of S. aureus strains and raw milk samples (Schlosser et al. 2007). 

Chatrathi, M.P, Wang, J., and Collins, G.E. (2007) Sandwich electrochemical immunoassay for the detection of Staphylococcal enterotoxin B based on immobilized thiolated antibodies. Biosens. Bioelectron., 22 (12), 2932. 

Wojciechowski J, Danley D, Cooptr J, Yazvenko N, Rowe Taitt C (2010) Multiplexed electrochemical detection of yersinia pestis and staphylococcal enterotoxin B using an antibody microarray. Sensors 10 3351-3362... 

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