Spleen ceUs

HAuCl -induced T-cell activation resulted in early cytokine gene expression by lymphocytes from both BN and LEW rats since two to four hour incubation with the metal was sufficient to induce an increase in IL-4 and IFN-y mRNA. Nevertheless, the expression of IL-4 predominated in BN T-ceUs while the expression of IFN-y was favoured in LEW T-ceUs. The in vitro findings correlated quite well with the profile of cytokine expression in spleen cells of BN and LEW rats injected with HAuCl, which gave relevance to the in vitro data. The pronounced over-expression of IL-4 induced by gold in BN rats was probably related to the fact that BN rats mount preferential Th2 responses whatever the stimulus. It would be interesting to determine the frequency of IL-4 producing T-cells upon stimulation with gold and whether this phenotype concerns a peculiar T-cell subset. 

In patients with hepatosplenic candidiasis, as the WBC count increases to more than 1000 ceUs/mm, imaging smdies can detect the presence of abscess or microabscesses in the liver and spleen, often found with acute suppurative and granulomatous reactions. 

Fig. 2.4 Monoclonal antibodies. After injecting the antigen and generating several clones of antibodies, the spleen containing B-cells is removed. Hybridoma cells are made by fusing spleen B-ceUs with a myeloma cell culture line. To isolate the individual hybridoma cells producing one clone of antibody, the mixed hybridoma culture is highly diluted and plated in 96-well plates with one cell or less per well...

Distribution TetracycUnes distribute widely throughout the body, including urine and prostate. They accumulate in reticuloendotheUal ceUs of the Uver, spleen, and bone marrow, and in bone, dentine, and enamel of unerupted teeth see below). Meningeal inflammation is not required for passage of tetracyclines into the cerebrospinal fluid (CSF). TetracycUnes cross the placenta and enter the fetal circulation and amniotic fluid. Relatively high concentrations are found in breast milk. 

Lymphatic The tissues and organs, including the bone marrow, spleen, thymus, and lymph nodes, that produce and store ceUs that fight infection and disease, [nih]... 

V-genes from IgM mRNA of B ceUs of immunized human donors periferal blood lymphocytes bone marrow spleen cclb... 

Count viable lymphoid cells in haemocytometer. Spleens frx>m immune mice yield about 10 cells, flum rats 3-5 x 10 cells, and the mesenteric nodes of rats, up to 2 X 10 ceUs. 

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