Specific DNA binding

It has now been found that the glucocorticoid response elements of mouse mammary tumour virus and the gene for chicken lysozyme also bind progesterone re- 

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Both lambda Cro and repressor proteins have a specific DNA-binding motif... 

One of the most important molecular functions of p53 is therefore to act as an activator of p21 transcription. The wild-type protein binds to specific DNA sequences, whereas tumor-derived p53 mutants are defective in sequence-specific DNA binding and consequently cannot activate the transcription of p5 3-con trolled genes. As we will see more than half of the over one thousand different mutations found in p53 involve amino acids which are directly or indirectly associated with DNA binding. 

The polypeptide chain of p53 is divided in three domains, each with its own function (Figure 9.16). Like many other transcription factors, p53 has an N-terminal activation domain followed by a DNA-binding domain, while the C-terminal 100 residues form an oligomerization domain involved in the formation of the p53 tetramers. Mutants lacking the C-terminal domain do not form tetramers, but the monomeric mutant molecules retain their sequence-specific DNA-binding properties in vitro. 

Decker, N., et al. Solution structure of the POU-specific DNA-binding domain of Oct-1. Nature 362 852-855, 1993. 

Rel homology domain (RHD) that encompasses a sequence-specific DNA-binding domain, a dimerization domain and a nuclear translocation signal (NLS) (Fig. la). RelA, cRel, and RelB contain a transcription activation domain (TAD). NF-kB 1 and NF-kB2 are synthesized as large precursors, pi 05 and pi 00, that are posttranslationnally processed to generate the mature forms, p50 and p52, which lack a TAD. 

Larson, J.S., Schuetz, T.J., Kingston, R.E. (1988). Activation in vitro of sequence specific DNA binding by a human regulatory factor. Nature 335, 372-375. 

The amino acid 58 was used in the solid-phase synthesis of sequence-specific DNA binding polyamides containing N-methylimidazole and N-methylpyrrole amino acids <96JACS6141> and it was also reported that the imidazole-acridine conjugate 59 could effectively catalyze the cleavage of t-RNA <96TL4417>. 

The availability of sequence-specific DNA binding molecules led to the development of bifunctional polyamides that covalently react with the minor groove of DNA. Two classes of alkylating agents were conjugated to the hairpin turn unit and bound proximal to their alkylation sites by a DNA-binding polyamide [58, 59]. 

Dervan, P. B. Design oe sequence-specific DNA-binding molecules. Science 1986, 232, 464-471. 

U. K. Laemmli. Specific gain- and loss-of-function phenotypes induced by satellite-specific DNA-binding dmgs fed to Drosophila melanogaster. Mol. Cell 2000, 6, 1013-1024. 

P. E. Efficient pH-independent sequence-specific DNA binding by pseudoisocyto-... 

Odenwald, W. F., Garbem, J., Amheiter, H., Toumier-Lasserve, E., and Lazzarini, R. A. (1989). The Hox-1.3 homeobox protein is a sequence-specific DNA-binding phos-phoprotein. Genes Dev. 3 158-172. 

High affinity, site specific DNA binding. DNA-dependent protein/protein interaction... 

Cogan JG, Sun S, Stoflet ES, Schmidt LJ, Getz MJ, Strauch AR 1995 Plasticity of vascular smooth muscle alpha-actin gene transcription. Characterization of multiple, single-, and double-strand specific DNA-binding proteins in myoblasts and fibroblasts. J Biol Chem 270 11310-11321... 

E7. Ehrlich, K. C., and Ehrlich, M., Highly repeated sites in the apolipoprotein(a) gene recognized by methylated DNA-binding protein, a sequence specific DNA-binding protein. Mol. Celt. Biol. 10, 4957-4960 (1990). 

Luo, J., et ah, Acetylation of p53 augments its site-specific DNA binding both in vitro and in vivo. Proc Natl Acad Sci USA, 2004, 101(8), 2259-64. 

Song, Z., et al., A novel cysteine-ridi sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex dass II genes via a repeated Cys-His domain and functions as a transcriptional repressor. / Exp Med, 1994, 180(5), 1763-74. 

Gagne et al, 2003). Some non-sequence-specific DNA-binding proteins, such as the linker histone HI, may even have a greater affinity for PAR than DNA (Malanga et al., 1998). Thus, an understanding of PAR is an important aspect of understanding the biology of PARP-1 and other PARPs. 

Klymenko T, Papp B, Fischle W, Kocher T, Schelder M, Fritsch C, Wild B, Wilm M, Muller J (2006) A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysinebinding activities. Genes Dev 20 1110-1122... 

Nucleolin together with HnRNP D has been shown to form the LRl transcription factor. LRl is a B cell-specific, sequence-specific DNA binding activity that regulates transcription in activated B cells (Hanakahi et at, 1997 Hanakahi and Maizels, 2000). DNA bending induced by nucleolin and hnRNP D might regulate the transcriptional activation by LRl (Hanakahi and Maizels, 2000). 

Gu W, Roeder RG (1997) Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90(4) 595-606... 

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