Src homology 3 domains

The Src homology 3 domain is a 60 amino acid long domain that binds to proline-rich sequences, thereby enabling protein-protein interactions. 

SH3 Src homology 3 domain Binding to prolin-rich sequences... 

Kato, M., Miyazawa, K., and Kitamuea, N. a deubiquitinating enzyme UBPY interacts with the Src homology 3 domain of Hrs-binding protein via a novel binding motif PX(V/I)(D/N)RXXKP, J Biol Chem, 2000, 275, 37481-7. 

V27CT, C-terminal domain of the Vps27 family SH3, Src-homology 3 domain gEAR, y-adaptin ear domain. The UIM motifs are shown as small black ellipses, the CAT domains as larger gray ones. 

Core antigen of hepatitis b virus Src homology 3 domain... 

Bunnel SC, Henry PA, Kolluri R, Kirchhausen T, Riekies RJ, Berg LI, Identification of ItklTsk Src Homology 3 domain ligands, J. Biol. Chem., 271 25646-25656, 1996. 

A more detailed picture of the folding of the SH3 (Src homology 3) domain of the Fyn protein kinase has been obtained by relaxation dispersion experiments.93 9 SFI3 domains bind proline-rich sequences and are key components of proteins involved in protein tyrosine kinase signalling pathways. The folding of the SFI3 domain of the Fyn protein kinase has been extensively characterized by stopped-flow and NMR experiments. CPMG relaxation dispersion analysis revealed that the Fyn SH3 domain is essentially a three-state folder with an intermediate state. 

S. Tanaka, T. Morishita, Y. Hashimoto, S. Hattori, S. Nakamura, M. Shibuya, et cd. C3G, a guanine nucleotide-releasing protein expressed ubiquitously, binds to the Src homology 3 domains of CRK and GRB2/ASH proteins. Proc Natl Acad Set, USA, 91 (8), 3443-3447, 1994. 

Figure 15.11. Modular Structure of Phospholipase C. The domain structures of three isoforms of phospholipase C reveal similarities and differences among the isoforms. Only the P isoform, with the G-protein-binding domain, can be stimulated directly by G proteins. For phospholipase Cy, the insertion of two SH2 (Src homology 2) domains and one SH3 (Src homology 3) domain splits the catalytic domain and a PH domain into two parts.

Generating Sequences for an Oncogenic Protein, the Src Homology 3 Domain... 

Yamabhai, M., and Kay, B. K. (1997). Examining the specificity of Src homology 3 domain-ligand interactions with alkaline phosphatase fusion proteins. Anal. Biochem. 247, 143-151. 

Knudsen, B. S., Feller, S. M., and Hanafiisa, H. (1994). Four proline-rich sequences of the guanine-nucleotide exchange fector C3G hind with unique specificity to the first Src homology 3 domain of Grk./. Biol. Chem. 269, 32781-32787. 

Identification of Itk/Tsk Src homology 3 domain ligands./. Biol. Chem. 271,... 

Shin, H., Hsueh, Y. P, Yang, F. C., Kim, E., and Sheng, M. (2000). An intramolecular interaction between Src homology 3 domain and guanylate kinase-like domain required for channel clustering hy postsynaptic density-95/SAP90./. Neurosci. 20, 3580-3587. 

Alexandropoulos, K., Cheng, G., and Baltimore, D. (1995). Proline-rich sequences that bind to Src homology 3 domains with individual specificities. Proc. Natl. Acad. Sci. USA 92,3110-3114. 

Karlsson, T, Songyang, Z., Landgren, E., Lavergne, C., Di Fiore, P. E, Anafi, M., Pawson, T, Candey, L. C., Claesson-Welsh, L., and Welsh, M. (1995). Molecular interactions of the Src homology 2 domain protein Shb with phosphotyrosine residues, tyrosine kinase receptors and Src homology 3 domain proteins. Oncogene 10, 1475-1483. 

Skare P, Kreivi JP, Bergstrom A, Karlsson R (2003) Profilin I colocalizes with speckles and Cajal bodies a possible role in pre-mRNA splicing. Exp Cell Res 286 12-21 Solomaha E, Szeto FL, Yousef MA, Palfrey HC (2005) Kinetics of Src homology 3 domain association with the proline-rich domain of dynamins specificity, occlusion, and the effects of phosphorylation. J Biol Chem 280 23147-23156... 

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