Sorbitol dehydrogenase

Figure 5. Example of dehydrogenase reactions which can be coupled with the bienzymatic bacterial bioluminescent system. ADH = alcohol dehydrogenase (EC 1.1.1.1), SDH = sorbitol dehydrogenase (EC 1.1.1.14), LDH = lactate dehydrogenase (EC 1.1.1.27), MDH = malate dehydrogenase (EC 1.1.1.37).

Sorbitol Sorbitol dehydrogenase, bacterial luciferase FMA-NADH 60... 

Chronic copper poisoning in domestic sheep is first characterized by a period of passive accumulation of copper in the tissues. This period varies from a few weeks to more than a year. During this time the animal appears outwardly normal although the liver may contain more than 1000 mg Cu/kg DW and plasma activities of aspartate transaminase, sorbitol dehydrogenase, lactic... 

The liver appeared to be a target organ for hexachloroethane following oral administration. When one dose of 500 mg/kg was administered in an olive oil aqueous emulsion to male sheep, the levels of glutamate dehydrogenase, sorbitol dehydrogenase, ornithine carbamyl transferase, and aspartate aminotransferase in serum increased in the 2-day period after compound administration and then normalized (Fowler 1969b). Hexachloroethane had no effect on bromsulphthalein uptake from the blood by liver cells, but the transfer of this dye to bile was reduced in sheep exposed to doses of 500-1,000 mg/kg/day. 

In clinical chemistry however, these systems have not been differentiated as yet. Since the oxidation of an alcohol corresponds to the reduction of a ketose or an aldose, the designations ketose reductase and aldose reductase, respectively, were suggested (H4, W14). In this paper however, the enzyme or enzyme system will be named polyol or sorbitol dehydrogenase (SDH), although the latter expression does not characterize exacdy the enzyme s function in a general biochemical sense. But sorbitol or fructose have been commonly used as substrates in clinical chemical investigations. 

Hepati c 10 (significant increases in serum sorbitol dehydrogenase) ... 

SDH = sorbitol dehydrogenase SGPT = serum glutamic pyruvic transaminase wk = week yr = year(s)... 

Fig. 6.7 A FAC-MS experiment for ligand ranking. Eight ligands for sorbitol dehydrogenase were infused, spanning a range of 2 pM to 8 nM [10]. All ligands were applied at 1 pM concentration, ensuring operation in the nonlinear region of the binding isotherm. Adapted with permission from Elsevier.

SORBITOL DEHYDROGENASE FRUCTOSE-1,6-BISPHOSPHATASE FRUCTOSE-2,6-BISPHOSPHATASE D-Fructose 2,6-bisphosphate, 6-PHOSPHOFRUCTO-2-KINASE Fructose-1,6-bisphosphate aldolase, ALDOLASE... 

RUBREDOXIN NAD REDUCTASE SERINE DEHYDROGENASE SORBITOL DEHYDROGENASE XANTHINE DEHYDROGENASE NAD formation,... 

SORBITOL DEHYDROGENASE POLYPHOSPHATE GLUCOKINASE POLYPHOSPHATE KINASE Polypodal attachment, BIOMINERALIZATION Polyribonucleotide synthase (ATP),... 

This MRL was calculated using a NOAEL of 1 mg/kg/day, based on the absence of detected adverse hepatic effects (Bruckner et al. 1986). Male Sprague-Dawley rats were exposed to 0, 1, 10 or 33 mg/kg/day, 5 days/weeks, for 12 weeks, by corn oil gavage. Slightly elevated blood levels of of sorbitol dehydrogenase and centrilobular vacuolation of the liver were observed at a LOAEL of 10 mg/kg/day, but not at 1 mg/kg/day. To accound for the study s intermittent exposure (5 days/week), the MRL was derived using an adjusted NOAEL of 0.71 mg/kg/day. Similar NOAEL and LOAEL values have been described for related hepatic effects in mice after exposure by gavage (Condie et al. 1986 Hayes et al. 1986). 

I.L. Kwee, H. Igarashi, T. Nakada, Aldose reductase and sorbitol dehydrogenase activities in diabetic brain In vivo kinetic studies using F-19 3-FDG NMR in rats, Neuroreport 7 (1996) 726-728. 

Adult male albino rats received 0, 500 or 1000 mg/kg di(2-ethylhexyl) phthalate by gavage for 15 days and were killed 24 h later (Siddiqui Srivastava, 1992). Relative testis weight and epididymal sperm counts were reduced at the highest dose, as was the activity of several enzymes relevant to spermatogenesis (aldose reductase and sorbitol dehydrogenase). The activity of testicular lactate dehydrogenase was increased at this dose. 

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