Somatotropin, recombinant

Somatotropin - recombinant, modified human growth hormone... 

Growth hormone (human) [CAS] Somatotropin, recombinant [SY] hGH, rDNA [SY] rhGH [SY]... 

Human growth hormone HGH 22.1 somatotropin, recombinant (Genotropin, Humatrope,... 

One technology uses bovine somatotropin (bST) produced by recombinant technology (38). Somatotropin [9002-72-6] is a growth hormone. The bST-supplemented cows provide an increase in milk output per cow or an increased feed efficiency. Recombinant bST, also known as recombinant bovine growth hormone (rBGH) is the synthetic analogue of a natural hormone that increases milk production in cows (39). The use of recombinant technology was approved by the FDA in 1993. The Commission of the European Community has recommended that the moratorium on commercial use of BGH be delayed until the year 2000. 

Some of these compounds could be considered as dietary additives, but various other terms, including pesticides, can also be used. They can have beneficial effects on the environment and this aspect will be discussed later. The ionophore monensin, which is an alicyclic polyether (Figure 1), is a secondary metabolite of Streptomyces and aids the prevention of coccidiosis in poultry. Monensin is used as a growth promoter in cattle and also to decrease methane production, but it is toxic to equine animals. " Its ability to act as an ionophore is dependent on its cyclic chelating effect on metal ions. ° The hormones bovine somatotropin (BST) and porcine somatotropin (PST), both of which are polypeptides, occur naturally in lactating cattle and pigs, respectively, but can also be produced synthetically using recombinant DNA methods and administered to such animals in order to increase milk yields and lean meat production. "... 

Capper, J. L., Castaneda-Gutierrez, E., Cady, R. A., and Bauman, D. E. (2008). The environmental impact of recombinant bovine somatotropin (rbST) use in dairy production. Proc. Natl. Acad. Sci. USA 105, 9668-9673. 

Hagemann et al. [3.13] calculated the absorption isotherms for recombinant bovine Somatotropin (rbSt) and found 5-8 g water in 100 g protein, which was not only on the surface but also inside the protein molecule. 

Recognition site mapping, 10 326 Recombinant bovine somatotropin, 13 2 Recombinant CHO cell products... 

There are many examples of ELIS As used for detecting host cell impurities in the literature. Pauly et al.12 developed an ELISA to detect impurities in erythropoietin that had a detection limit of around 0.05 ng/ml. SDS polyacrylamide gel and Western blot analysis were used to confirm the spectrum of proteins detected and to demonstrate the specificity of the antibody preparation. Anicetti et al.14 describe an assay for the detection of E. coli proteins in recombinant DNA-derived human growth hormone. Whitmire and Eaton15 report on an immuno-ligand assay for quantitation of process-specific E. coli host cell contaminant proteins in a recombinant bovine somatotropin. 

Whitmire, M.L. and L.C. Eaton (1997). An immunoligand assay for quantitation of process specific Escherichia coli host cell contaminant proteins in a recombinant bovine somatotropin. J Immunoassay 18(1) 49-65. 

Recombinant bovine growth hormone (bovine somatotropin, BST)... 

Milk residue studies demonstrated that use of recombinantly derived somatotropin, even at exaggerated doses, did not lead to concentrations of bovine somatotropin in milk above those normally present in the untreated cows (0.9-1.6 ppb) (52, 53). Bovine somatotropin occurs naturally in cow milk at variable levels generally less than 2 ppb, but they may occasionally range up to 10 ppb. 

In regard to tissue residue data, recombinantly derived somatotropin treatment of cows leads, at most, to a doubling of bovine somatotropin concentrations to levels of 4.2 ppb in muscle and 25 ppb in liver. Cows treated with recombinantly derived somatotropin further show an up to doubling in IGF-I levels in muscle and liver. 

Porcine somatotropin and poultry somatotropin are both under development for use in swine and poultry. Recombinantly produced porcine somatotropin has been found to enhance performance dramatically. Increases in protein deposition, decreases in fat deposition, and depression of voluntary feed intake after injections of recombinant porcine somatotropin have been found across breeds. 

This polypeptide hormone constitutes one form of the recombinant bovine somatotropins used to increase milk production in dairy cows. Several studies have been performed in the United States, United Kingdom, and Germany to determine the concentrations of this drug in milk (99, 100). 

Application of recombinant DNA technology to clone and express the gene for bovine somatotropin has made it possible to obtain an unlimited amount of the hormone and provides further an opportunity to investigate benefits that may be derived from controlling milk production through supplemental administration of bovine somatotropin on a commercial scale. Central to studies conducted to investigate the safety and efficacy of recombinant-derived bovine somatotropin is the need for a sensitive analytical method that could be used to estimate bovine somatotropin levels in various biological fluids. 

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