Sodium Diethylstilbestrol

C1H03S 7790-94-5) see Actinoquinol Azosemide Bendroflumethiazide Clopamide Diclofenamide Diethylstilbestrol disulfate Dorzolamide Furosemide Glibenclamide Glimepiride Hydroflumethiazide Lomoxicam Mafenide Meticrane Metolazone Saccharin Sildenafil Sodium picosulfate Sulfanilamide Tiotixene Tripamide Xipamide... 

An alternative cleanup procedure is the partition of the raw extract, which often contains considerable amounts of lipid material, between an organic and an aqueous sodium hydroxide phase. With this partitioning scheme, the analytes are further fractionated into estrogens and nonestrogens. The presence of phenolic groups in the molecules of estrogens such as diethylstilbestrol and zeranol ensures their complete extraction from organic phases such as chloroform or tert.-butyl methyl ether into the aqueous sodium hydroxide phase (435, 438, 447). Further purification could be accomplished by neutralization of the sodium hydroxide solution and back-extraction of the contained diethylstilbestrol into diethyl ether (435), or adjustment of the pH of the sodium hydroxide solution to 10.6-10.8 and back-extraction of the contained zeranol into a chloroform phase (447). 

Methanococcus voltae contains a membrane-bound vanadate-sensitive ATPase [48] that is inhibited by diethylstilbestrol, an inhibitor of eukaryotic P-type ATPases. The purified enzyme is composed of a single subunit (Mr 74 000), forms a covalent acyl-phosphate enzyme intermediate, and is not inhibited by nitrate or bafilomycin [49]. No such ATPase activity has been reported in other archaea. The presence of a second ATPase in M. voltae has been inferred since membranes react with antiserum prepared against the 3 subunit from the V-type ATPase of S. acidocaldarius [50]. Two peptides are detected whose Mr values (51 000 and 65 000) correspond to the masses for the two laigest subunits of the S. acidocaldarius ATPase [51]. There is evidence that ATP synthesis in the M. voltae enzyme is due to the operation of a sodium-translocating ATPase [50]. The relationship of the putative V-like ATPase to the sodium-translocating ATPase has not been established. 

The use of HPLC-ED in the analysis of the anabolic steroids and metabolites, diethylstilbestrol, taleranol, zearalenol, zearalenone and zeranol (Figure 7.5), in mammalian tissue has been discussed.Diethylstilbestrol has been measured in animal tissue using an ODS-modified silica column with methanol-aq. phosphate buffer (50 mmol L pH 3.5) (67 + 33) as eluent and ED (GCE, +0.9 V vs Ag/ AgCl). Sample homogenisation was followed by LLE into MTBE, back-extraction into aqueous sodium hydroxide and SPE (ODS-modified silica). The LoD was approximately 0.5 pg kg wet weight (10 g sample). Clenbuterol assay in calf urine was discussed above (Chapter 6, Section 2). 

Flock and Bollman (52) investigated phosphatide turnover following administration of diethylstilbestrol to cocks. When radioactive sodium phosphate was given intraperitoneally to cocks, labeled phosphatides ap-... 

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