Single duplex DNA

Mel nikov, S.M., Sergeyev, V.G. and Yoshikawa, K. (1997c) Cooperation between salt induced coil-globule transition in single duplex DNA complexed with cationic surfactant and sphere-rod transition of surfactant micelles. Prog. Colloid. Polym. Sci., 106, 209-214. 

Mel nikov, S.M. and Yoshikawa, K. (1997d) First-order phase transition in large single duplex DNA induced by a nonionic surfactant. Biochem. Biophys. Res. Commun., 230, 514-517. 

Murayama, H. and Yoshikawa, K. (1999) Thermodynamics of the collapsing phase transition in a single duplex DNA molecule. J. Phys. Chem. B., 103, 10517-10523. 

Yoshikawa, K., Kidoaki, S., Takahashi, M., Vasilevskaya, V.V. and Khokhlov, A.R. (1996b) Marked discretness on the coil-globule transition of single duplex DNA. Ber. Bunsen-Ges. Phys. Chem., 100, 876-880. 

Fore, S., Yuen, Y., Hesselink, L., and Huser, T. (2007). Pulsed-Interleaved Excitation FRET Measurements on Single Duplex DNA Molecules Inside C-Shaped Nanoapertures. Nano Lett. 7,1749-1756. 

Takahashi M, Yoshikawa K, Vasilevskaya VV, Khokhlov AR. Discrete coil-globule transition of single duplex DNAs induced by polyamines. J. Phys. Chem. 1997 101 9396-9401. 

Cooperation between salt induced globulecomplexed with cationic surfactant and sphorofod transition of surfactant micelles... 

Key words Single duplex DNA -cationic surfactant - fluorescence microscopy - coil-globule transition -sphere-rod transition of micelles... 

Not all DNAs are double helices duplex DNA) Some types of viral DNA are single stranded and even a few DNA triple and quadruple helices are known... 

A graph of the fraction of single-stranded DNA reannealed c/cq) as a function of CqI on a semilogarithmic plot is referred to as a c t (pronounced cot ) curve (Figure 12.20). The rate of reassociation can be followed spectrophoto-metrically by the UV absorbance decrease as duplex DNA is formed. Note that... 

If DNA from two different species are mixed, denatured, and allowed to cool slowly so that reannealing can occur, artificial hybrid duplexes may form, provided the DNA from one species is similar in nucleotide sequence to the DNA of the other. The degree of hybridization is a measure of the sequence similarity or relatedness between the two species. Depending on the conditions of the experiment, about 25% of the DNA from a human forms hybrids with mouse DNA, implying that some of the nucleotide sequences (genes) in humans are very similar to those in mice. Mixed RNA DNA hybrids can be created in vitro if single-stranded DNA is allowed to anneal with RNA copies of itself, such as those formed when genes are transcribed into mRNA molecules. 

ASON are sequences of usually 17-30 bases of single-stranded DNA that hybridize to specific genes or their mRNA products by Watson-Crick base pairing and disrupt their function. In the case of AS-ODN (antisense oligodeoxyribonucleotides) cellular RNAseH is able to bind to the DNA-RNA duplex and hydrolyze the RNA, resulting in increased transcript turnover. Modifications to the deoxy moiety at the 2 -sugar position prohibits RNAse H action. 

Streptavidin-single-stranded DNA covalent conjugates were described as the building blocks for assembling nanostructured scaffolds [31], The amount and type of biotinylated ligands were used to modulate the affinity of duplex formation between solid-phase-bound nucleic acid templates and DNA-streptavidin conjugates. This system has been proposed for the design of fine-tuned sequence detection systems. 

Bacterial plasmids are small, circular, duplex DNA molecules whose natural function is to confer antibiotic resistance to the host cell. Plasmids have several properties that make them extremely useful as cloning vectors. They exist as single or multiple copies within the bacterium and replicate independently from the bacterial DNA. The complete DNA sequence of many plasmids is known hence, the precise location of restriction enzyme... 

Autoradiography The detection of radioactive molecules (eg, DNA, RNA, protein) by visualization of their effects on photographic film. Bacteriophage A virus that infects a bacterium. Blunt-ended DNA Two strands of a DNA duplex having ends that are flush with each other. cDNA A single-stranded DNA molecule that is complementary to an mRNA molecule and is synthesized from it by the action of reverse transcriptase. 

Lewis, FD, Wasielewski MR (2004) Dynamics and Equilibrium for Single Step Hole Transport Processes in Duplex DNA. 236 45-65... 

---