Silica gel membranes

Several standard DNA isolation kits are commercially available, including the QIAamp DNA Stool Mini Kit and the DNeasy Plant Mini Kit made by Qiagen. Both of these products are based on silica gel membrane technology and allow for the extraction of total DNA from processed foods and raw foodstuffs, respectively. In... 

A recent development is to combine filtration with solid-phase extraction separation. These filter modules contain a unique silica gel membrane that binds up to 20 pg of DNA in the presence of a high concentration of chao-tropic salt and allow eventual elution in a small volume of low-salt buffer. They also contain an asymmetric laminar membrane with a gradation of pore sizes for efficient removal of material precipitated in the lysate. Such membrane filters eliminate time-consuming phenol-chloroform extraction and alcohol precipitation. The impregnation of silica in the membrane matrix also prevents the problems associated with loose resins and slurries. High-purity plasmid DNA eluted from such modules is ready to use and often needs no further precipitation, concentration, or desalting. 

Zhao, C.-Z., Egashira, N., Kurauchi, Y., Ohga, K. Electrochemiluminescence sensor having a pt electrode coated with a ru(bpy)(3)(2-modified chitosan silica-gel membrane. Anal. Sd. 14, 439-441 (1998)... 

To elute purified RNA, transfer the RNeasy column to a new 2-mL collection tube, pipet 40 pL of RNase-free water directly onto the silica-gel membrane, and centrifuge at >8000 x g for 1 min. Run eluted RNA over the same column again to increase RNA yield. 

Thermogravimetric Analysis - The thermogravimetric analysis of silica-gel particles (without TMPTA) indicates a good thermal resistance (above 320°C) of silica-gel membranes -Figure 18. 

PRO-TEK Colorimetric Air Monitoring Badge System Polyvinyl chloride filter Silver membrane filter Silica gel tube... 

To keep the consumption of the valuable platinum low, thin foils have been glued to a graphite support [34] or thin-layers of platinum have been sputtered on to a glass base [59]. Platinum can be used in a particle electrode by plating silica gel with platinum [60], or in a solid polymer electrolyte where platinum is incorporated into a nafion ion exchange membrane [61]. 

The new lipid occurred only in the plasma hpids of newborns and was not present in membrane hpids of red cell membranes or platelets. Total lipids were extracted from plasma and from red blood cell membranes and platelets. A total lipid profile was obtained by a three-directional PLC using silica gel plates and was developed consecutively in the following solvent mixtures (1) chloroform-methanol-concen-trated ammonium hydroxide (65 25 5, v/v), (2) chloroform-acetone-methanol-ace-tic acid-water (50 20 10 15 5, v/v), and (3) hexane-diethyl ether-acetic acid (80 20 1, v/v). Each spot was scraped off the plate a known amount of methyl heptadecanoate was added, followed by methylation and analysis by GC/MS. The accmate characterization of the new lipid was realized using NMR technique. 

Phospholipids in purple membrane of Halobacterium Silica gel G halobium Note H = hexane, D = diethyl ether, F = formic acid, BuOH = butanol. 23... 

Ethanol and choline glycerolipids were isolated from calf brain and beef heart lipids by PTLC using silica gel H plates. Pure ethanol amine and choline plasmalogens were obtained with a yield of 80% [74]. Four phosphohpid components in the purple membrane (Bacteriorhodopsin) of Halobacterium halobium were isolated and identified by PTLC. Separated phosphohpids were add-hydrolyzed and further analyzed by GC. Silica gel G pates were used to fractionate alkylglycerol according to the number of carbon atoms in the aliphatic moiety [24]. Sterol esters, wax esters, free sterols, and polar lipids in dogskin hpids were separated by PTLC. The fatty acid composition of each group was determined by GC. 

See also Colloidal silica gels Colloidal graphite, 12 795 Colloidal liquids, 7 294-295 Colloidal materials, as membrane foulants, 21 664... 

Some bead materials possess porous structure and, therefore, have very high surface to volume ratio. The examples include silica-gel, controlled pore glass, and zeolite beads. These inorganic materials are made use of to design gas sensors. Indicators are usually adsorbed on the surface and the beads are then dispersed in a permeation-selective membrane (usually silicone rubbers). Such sensors possess high sensitivity to oxygen and a fast response in the gas phase but can be rather slow in the aqueous phase since the gas contained in the pores needs to be exchanged. Porous polymeric materials are rarer and have not been used so far in optical nanosensors. 

Miyake, N., Wainright, J. S. and Savinell, R. E 2001. Evaluation of a sol-gel derived Nafion/silica hybrid membrane for proton electrolyte membrane fuel cell applications. I. Proton conductivity and water content. Journal of the Electrochemical Society 148 A898-A904. 

Abstract We review various methods for the photochemical grafting of organic polymers to various substrates including, organic films, membranes, planar gold, silicon wafers, glass, silica gel, silica nanoparticles, and polydimethylsiloxane micro-channels. An emphasis is placed on photoinitiated synthesis of polymer brushes from planar gold and silicon. 

Fig. 1.5.3 Aerosol generator assembly for the production of composite or coated particles (a) carrier gas (e.g., helium) tank, (b) drying column containing silica gel and molecular sieve, (c) Millipore membrane of 0.1 p.m pore size, (d) flow meters, (e) nuclei (e.g., NaCI, AgCI) generator, (f) and (m) boilers containing different reactant liquids, (g) and (n) condensation chambers, (h) heater, (i) chamber for recondensation of droplets, (q) and (e) coreactant containers, (k) and (p) vapor injection ports, (I) and (g) reaction chambers, (r) powder collector. (From Ref. 39.)...

Various types of detector tubes have been devised. The NIOSH standard number S-311 employs a tube filled with 420—840 pm (20/40 mesh) activated charcoal. A known volume of air is passed through the tube by either a handheld or vacuum pump. Carbon disulfide is used as the desorbing solvent and the solution is then analyzed by gc using a flame-ionization detector (88). Other adsorbents such as silica gel and desorbents such as acetone have been employed. Passive (diffuse samplers) have also been developed. Passive samplers are useful for determining the time-weighted average (TWA) concentration of benzene vapor (89). Passive dosimeters allow permeation or diffusion-controlled mass transport across a membrane or adsorbent bed, ie, activated charcoal. The activated charcoal is removed, extracted with solvent, and analyzed by gc. Passive dosimeters with instant readout capability have also been devised (85). 

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