Immunocytochemical labeling

Figure 18. (a) Immunocytochemical labeling of B-lymphocytes in muscle from a patient with juvenile dermatomyositis. (b) Immunocytochemical labeling of T8 lymphocytes showing that the infiltrate contains very few cells of this type (serial section). 

Figure 20. Immunocytochemical labeling of T8 lymphocytes showing some cells in close contact with muscle fiber plasma membranes (arrowheads).

Krenacs, T., Krenacs, L., BozUky, B., and Ivanyi, B. (1990) Donble and triple immunocytochemical labeling at a light microscopic level in histopathology. Histochem. J. 22, 530-536. 

Immunocytochemical Labeling. Antibodies were used as post-embedding markers. Sections of decayed wood were first incubated on a drop of TBS (Tris-phosphate saline buffer 0.1 M, pH 7.4, NaCl 0.15 M or 0.5 M), glycine... 

The grid is now ready for repeating the immunocytochemical labeling using a primary Ab directed against another antigen. The procedure is performed exactly as described in Section 3.1. 

Nakane, P. R. and Pierce, G. B., Jr. (1966) Enzyme-labelled antibodies. Preparation and applicauon for the localization of antigens./. Hislochem. Cytochem. 14, 929-931. StembCTger, L. A. (1979) Immunocytochemistry, 2nd Ed., John Wley, New York. Mason, D. Y. (1985) Immunocytochemical labelling of monoclonal anubodies by the APAAP immunoalkaline phosphatase technique, in Techntquestnimmunocylochermstry, Vol. 3 (Bullock, G. R. and Petrusz, P., eds.), Academic, London, pp. 25-42. 

It may be noted that, besides lEM double labeling, the EM-silver enhancement per se is also useful when lEM preparations are to be photographed at low magnifications. The immunocytochemical labeling can be... 

In the nucleus accumbens immunocytochemical labelling shows that, in contrast to the hippocampus, CBi receptors are located on glutamatergic nerve terminals (Robbe et al. 2001). In a mouse brain slice preparation, low-frequency stimulation (13 Hz for 10 min) evoked LTD of field EPSPs or EPSCs recorded in the nucleus accumbens (Robbe et al. 2002,2003). This LTD apparently depended on the activity of endocannabinoids, since it was occluded by 0.3 pM WIN55,212-2, blocked by 0.1-1 pM rimonabant or 2 pM AM251, and critically, it was absent in CBr " mice. While the use of CBr " mice shows that CBi receptors are essential for this form of synaptic plasticity in the nucleus accumbens, the cannabinoid ligands were not tested in CBr " animals, and so although it is likely, it is not certain that they produced their effects via the CBi receptor. In a similar study, low-frequency stimulation (10 Hz for 5 min) induced LTD of the population spike recorded in... 

A third type of aspiny striatal interneuron is identified by its immunocytochemical labeling with somatostatin, neuropeptide Y, and NADPH diaphorase. These cells have also been shown to be distinguishable from parvalbumin/GABA interneurons on the basis of morphological and physiological criteria (Kawaguchi 1993). Somatostatin positive synapses are formed mainly on shafts of dendrites and dendritic spines of spiny neurons (Takagi et al. 1983). As before, all the spines involved in this connection receive another asymmetrical synaptic contact. 

Fig. 9. Calcium binding proteins in the MOB. A. Distribution of cells labeled with an antibody to Calbindin (CALB) using immunocytochemistry. B. Parvalbumin (PARV) immunocytochemically labeled cells are found mainly in the EPL in the bulb. C. Golgi stained section showing impregnated external tufted cells. D. An example of how cells may be identified by other methods. In this case an external tufted cell is shown after intracellular injection of the marker biocytin. Bar in D, /tm.

Immunolabeling of cultured cells grown on coverslips is a relatively simple process, especially if monolayers are being used. For detailed descriptions of how to grow cells on coverslips for subsequent immunocytochemical labeling, see ref. 10. Because the cells do not have any cut surfaces, they must be permeabilized this may be accomplished by adding Tnton X-100 (to 0.2%, v/v) to all buffers Alternatively, permeabilize the cells by immersing coverslips in cold methanol (4°C) for 10 min. Thereafter, rehydrate the cells with PBS. Tnton X-100 may still be mcluded in buffers if required. 

Immunocytochemical labeling methods and related techniques for ultrastmctural analysis of neuronal connectivity. In Merighi A, Carmignoto G (eds) CeUular and molecular methods in neuroscience research. Springer, New York, pp 161-180... 

Immunocytochemistry and Kelated Techniques is a collection of protocols for the immunocytochemical analysis of neurons and neural networks where emphasis is given not only to the immunostaining protocol per se but also to its possible ameliorations in qualitative and quantitative terms, and to the possibility of employing immunocytochemical labeling as a part of a more comprehensive approach to understand the structure and function of the brain. 

Blanchette, R. A., Abad, A. R., Farrell, R. L., Leathers, R. L. (1989). Detection of lignin peroxidase and xylanase by immunocytochemical labeling in wood decayed by basidiomycetes. Appl. Environ. Microbial. 55,1457-1465. 

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