Correlative microscopy

Terry, B. R., Matthews, E. K., and Haseloff, J. (1995). Molecular characterization of recombinant green fluorescent protein by fluorescent correlation microscopy. Biochem. Biophys. Res. Commun. 217 21—27. 

Goedhart, J, Hink, M. A, Visser, A. J, Bisseling, T. and Gadella, T. W, Jr. (2000). In vivo fluorescence correlation microscopy (FCM) reveals accumulation and immobilization of Nod factors in root hair cell walls. Plant J. 21, 109-19. 

Van der Voort HTM, Valkenburg JAC, Van Spronsen EA, Woldringh CL, Braken-hoff G J. Confocal microscopy in comparison with electron and conventional microscopy, in Correlative Microscopy in Biology. Instrumentation and Methods (Hayat MA, ed.), Academic Press, Orlando, FL, 1987, pp. 60-81. 

Robinson, J. M., Takizawa, T., and Vandre, D. D. (2000) Applications of gold cluster compounds in immunocytochemistry and correlative microscopy comparison with colloidal gold. /. Microsc. 199, 163-179. 

Takizawa, T., Suzuki, K., and Robinson, J. M. (1998) Correlative microscopy using FluoroNanogold on ultrathin cryosections proof of principle. J. Histochem. Cytochem. 46, 1097-1102. 

Figure 3 Cross-correlation microscopy to study protein dynamics at the plasma membrane, (a) Methodology for analyzing protein dynamics at the plasma membrane. Cells are transiently transfected with the fluorescently labeled protein of interest. The antigen receptor and plasma membrane are also labeled with fluorescent markers. Plasma membrane masks generated for each time point are used to quantify protein dynamics at the plasma membrane over the time course. Representative traces of two cells that express GFP-PLCy-(SH2)2 showing (b) recmitment to the plasma membrane and (c) interactions with clustered IgE receptors after stimulation as described in Reference 61.

Wiseman PW, Brown CM, Webb DJ, Hebert B, Johnson NL, Squier JA, Ellisman MH, Horwitz AF. Spatial mapping of in-tegrin interactions and dynamics during cell migration by image correlation microscopy. J. Cell Sci. 2004 117 5521-5534. 

Robinson J.M. Meeting report Biological labeling and correlative microscopy. Acta Histochem. 103, 2001, 261-264. 

Alexandrakis G, Brown EB, Tong RT, McKee TD, Campbell RB, Boucher Y, Jain RK (2004) Two-photon fluorescence correlation microscopy reveals the two-phase nature of transport in tumors. Nat Med 10 203— 207... 

Takizawa T, Robinson JM (2000) Analysis of antiphotobleaching reagents for use with FluoroNanogold in correlative microscopy. J Histochem Cytochem 48 433 136... 

Key Words Transmission electron microscopy scanning electron microscopy Gram-positive Gram-negative immune labeling histochemistry colloidal gold quantum dots correlative microscopy fluorescence. 

Correlative microscopy involves multiple separate microscopy platforms, which are correlated to view the same exact structure of interest with complimentary information [10]. Such systems allow structure-function relationships to be determined at high resolutions. Examples of correlative microscopy include light and electron microscopy, light and atomic force microscopy, immunocytochemistry, and histochemistry, etc. 

Modla S, Czymmek KJ (2011) Correlative microscopy a powerful tool for exploring neurological cells and tissues. Micron 42 773-792... 

Hayat MA (ed.) (1987) Correlative Microscopy in Biology -Instrumentation and Methods. Orlando, FL Academic Press. 

Membrane protein detection by image correlation microscopy... 

Complementary microscopy has been stressed as being essential in understanding the nature of polymer structure, because of the common formation of artifacts that can result in misinterpretation of the image information. Carter and Harb [8] used the term correlative microscopy, stating that subjecting a specimen to a variety... 

Correlative microscopy providing new understanding in the biomedical and plant sciences. Micron 43 565-582... 

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