Sera proteomic study

Example Sera proteomic study. Here is an example of a sample collection protocol that is being used for sera proteomics study in a hospital. 

The sample materials from which proteins for proteomics studies may be extracted include fresh or snap-frozen cells from varied sources such as biological fluids, (serum, urine, plasma) and solid tissues such as biopsy specimens. Moreover, proteins isolated from ethanol-fixed paraffin-embedded tissues can be utilized for MS analysis.2 Protocols for the identification of proteins from formalin-fixed paraffin-embedded (FFPE) tissues have been recently developed.3 4 FFPE materials are the most common forms of biopsy archives utilized worldwide, and represent an important advancement for the large-scale interrogation of proteins in archival patient-derived materials. Finally, laser capture microdissected tissues have been successfully used for MS analysis.45... 

The term proteome means the total protein complement of a genome, and pro-teomics means the analysis for proteome. The combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) is a proteomic method of high-throughput analysis of protein expression. By using this 2-DE and MS, proteomic studies have identified many proteins that may be involved in the pathogenic mechanism of cancers. These studies analyzed cancer cell lines, as well as cancer tissues or serum from patients. 

Any proteomic study starts with the collection of proteins from biological samples such as cell culture media, cultured cells, serum, or any biological fluid, and a variety of animal tissues. The first step is to obtain a protein sample under conditions of least protein degradation. This involves use of various protease inhibitors that stop the protein degradation. The use of protease inhibitors depends on the type of sample and the analytical technique used in the subsequent analysis. The selection of protease inhibitors used is critical since many protease inhibitors and detergents used in the preparation of tissue homogenates can interfere with mass spectrometry (MS)... 

Wong N, et al. Prediction of Ever fibrosis and cirrhosis in chronic hepatitis B infection by serum proteomic fingerprinting A pilot study. Clin Chem 2005 51 In Press. 

Corbett BA, Kantor AB, Schulman H, Walker WL, Lit L, Ashwood P, Rocke DM, Sharp FR (2007) A proteomic study of serum from children with autism showing differential expression of apolipoproteins and complement proteins. Mol Psychiatry 12 292-306... 

A detailed proteomic study of plasma and serum has been carried out in different laboratories primarily in the United States under the sponsorship of HUPO. 

Finally, it must be mentioned that significant proteomic studies have been published dealing with the modifications of serum protein expression that may occur in healthy as well as in animals presenting with various experimental diseases (Eberini et al, 1999 Ebrini et al, 2000 Gianazza et al., 2002 Haynes etal, 1998 Miller etal., 1998 Miller etal., 1999 Wait et al., 2001 Wait et al., 2002). 

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