Sequencing projects

The primary data of sequencing projects are DNA sequences. These become only really valuable through their annotation. Several layers of analysis with bioinformatics tools are necessary to arrive from a raw DNA sequence at an annotated protein sequences ... 

In the biosciences, a databank (or data bank) is a structured set of raw data, most notably DNA sequences from sequencing projects (e.g. the EMBL and GenBank databases). 

With the onset of genomic biology, there are now many sequences derived from genome sequencing projects that are too divergent to be considered species variants of known peptidases. Of the 54,124 sequences in the MEROPS database only 18,741 (34.6%) have been assigned to an identifier. 

The second and most important finding of this sequencing project was that the difference of 14 Da between the two proteins was not because of a methy-lation of the protein isolated from chicken but because of a substitution of valine for leucine. The MS/MS analysis of the tryptic pieces in which the substitution occurred is shown in Figure 10.11. Had this been a bottom-up... 

The availability of data from the public sequencing projects has enabled the use of nucleotide sequence information from genomes and ESTs to complement biochemical approaches, and to assemble a new, clearer working map of plant Ci... 

Sequencing, of fermentation, 11 40-41 Sequencing project results, assembly and analysis of, 12 510-512 Sequential function charts (SFC), 20 705-707... 

All predicted protein sequences lacking any significant sequence similarity to characterized proteins are labeled hypothetical proteins. The majority of these cases come from the genome sequencing projects. Example ... 

WORMPEP Caenorhabditis elegans genome sequencing project protein database (WormPep)... 

This chapter anticipates the completion of Arabidopsis thaliana, Drosophila melanogaster, and Homo sapiens genome sequencing projects by reviewing current ideas of the evolution of sequence families. In parallel the related issue of domain homolog detection is discussed in light of continuing efforts to map the complete set of domain families. 

Rat Genome Sequencing Project Gonsortium. 2004. Genome sequence of the Brown Norway rat yields insights into mammalian evolution. Nature 428 493. 

In a DNA sequencing project, an open reading frame (ORF) has been identified. The nucleotide sequence includes a coding region for an SH2 domain in the protein product. This potential protein is most likely to... 

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