Sequence region

SH2 domains generally consist of approximately 100 amino acid residues [49,50, 60,61 ] and have been first identified as a conserved sequence region between the oncoproteins Src and Fps [19, 48]. By means of sequence homology, SH2 domains have been uncovered in numerous other intracellular signal transduction proteins (Figs. 1 and 4) [20]. 

The term homology domain or functional domain should be used only for those protein regions that either are known to be domains in the structural sense or that are at least predicted to fulfil that condition. Conserved sequence regions that are too short to fold independently of the rest of the protein should rather be referred to as motifs . A considerable number of those functional motifs have important roles, e.g. by being responsible for specific domain- or protein-recognition events. 

TapCT The C-terminus of the mammalian nuclear RNA export factor NXFl/2 (also known as Tap) contains a sequence region with significant similarity to UBA-like domains. This region is also found in the yeast RNA export factor Mex67. A three-dimensional structure of this domain is available and confirms its similarity to the UBA domain [68]. This UBA-like domain does not appear to bind to ubiquitin but rather to the Phe-Gly repeat motif found in a number of nu-cleoporins. The interaction surface of the UBA-like TapCT domain with a Phe-Gly-containing loop was mapped by an NMR/X-Ray combination technique and shown to be different from the ubiquitin-binding mode the Phe-Gly loop binds on the backside of the UBA-like domain and is in contact with helices a2 and a3 [68]. 

SELEX is a widely used technique for screening of aptamers which are nucleic acid ligands. According to this method, a pool of DNA with a random sequence region attached to a constant chain is constituted by amplification then transcribed to RNA. RNA pool is separated according to the affinity of RNA molecules to a target protein. DNA molecules obtained by reverse transcription from retarded RNA molecules are amplified and the cycle is repeated. 

Regulatory sequence Region of DNA responsible for regulating the transcription of the gene. 

The discovery of sequence alterations can be accomplished by the creation of mismatched base pairs, which occur when a test sample containing a sequence alteration is denatured and re-annealed with an otherwise complementary normal control sequence. The routine and specific discovery of these mismatches and their localization to within an easily sequenced region is the goal of several techniques. There are two general methods for identification of mismatched sequences ... 

In this section approximately aligned sequences from the promoter core region are presented and analyzed. The alignments are based on the A-T-rich box centered about 26 nucleotides upstream from the transcriptional start site and they are presented in Table 4. Sequence regions extending approximately from positions —50 to +10 are analyzed. 

The 3 ends of transcripts are frequently ragged and map to one sequence region or to closely spaced regions. For a few of the stable RNA genes the termination site mapped to... 

B. CNBr cleavage of H202-oxidixed dimer at Met Five methionines, i.e., Mefl, Met 7, Met, Met, and Met, are in the rhSCF sequence, with Met and Met in the N-terminal loop created by the Cys -Cys bond and Met S in a sequence region shared by both disulfide loops. A complete... 

One disorder of mitochondrial transport in humans is due to a failure to transport the methylmalonyl-CoA-mutase precursor protein. The mutation responsible for this disorder affects the signal sequence region. An-... 

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