Amino acid sequences hypervariable regions, human

FIGURE 26.2 (See color insert following page 40). The differences from a murine antibody (a) to a fully human antibody (d). In (b), the constant regions of the murine antibody have been replaced with human constant domains. In (c), the amino acid sequences adjacent to the three hypervariable regions in the variable domains had been humanized by modeling amino acid substitutions that are more commonly found in human antibodies. 

The V regions of eight of the twelve X chains appear to have identical amino acid sequences two differ by one residue from these eight, one protein differs by two residues, and the other by three. Of the variations observed in the four proteins, all occurred at or near positions 25, 52, or 97, which are in or near hypervariable regions of human or mouse K, and of human X chains, and which are known to be associated with the antibody combining site. 

D. Davis, D. M. Stephens, C. Willers, P. J. Lachmann, Glycosylation governs the binding of antipeptide antibodies to regions of hypervariable amino acid sequence within recombinant gpl20 of human immunodeficiency virus type 1. J. Gen. Virol. 1990, 71, 2889-2898. 

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