Sephadex beads

The gel media should be hydrated and washed with a buffer system compatible with maintaining the protein target in its native state. To thoroughly remove contaminants present in the gels, multiple washes are necessary. Sephadex beads... 

Catalase is the largest protein in this set and would be excluded completely from the Sephadex beads (exclusion limit approx. 200,000). That means that to the catalase molecules, the beads appear to be solid, and so the catalase molecules can only dissolve in the fluid outside the beads (the void volume). They would be eluted from the column when a volume of eluent equal to this void volume had passed through the column. 

A considerable amount of experience has been gained with a wide variety of materials in the preparation of affinity media, for example, polystyrene beads, cellulose, Sephadex, bead form polyacrylamides, bead form agaroses, and glass beads. With such a wide range of supports available, it would be unwise to recommend any one support for all applications. However, the bead form agaroses display most of the... 

The media most commonly used in gel chromatography (otherwise termed gel filtration or molecular sieve chromatography) are the cross-linked dextrans (Sephadex), bead-form polyacrylamides (Bio-Gel P) and the bead form agaroses (Sepharose, Bio-Gel A, and Indubiose). It is on these gels that most data have been accumulated and the majority of interactions of interest occur. 

Sephadex beads for gel filtration ion-exchange Sephadex resins... 

No bacterial replication was detected at inflammatory sites caused by injection of Sephadex. We generated artificial inflammation in mice with GI-IOIA tumors by s.c. implantation of Sephadex beads (see material and methods for experimental details). After i.v. delivery of bacteria, no light emission was seen at the Sephadex implantation site, even though Sephadex-induced inflammation was apparent. However, in the same animal, the tumor was colonized with luminescent bacteria, indicating the importance of a suppressed lymphatic system for bacterial survival and replication. 

Remove the excess water from above the Sephadex beads in the graduated cylinder with a syringe. 

MIP-la and CCR2 were deleted from mice using homologous recombination techniques. Mice were sensitized to PPD from Mycobacteria or Schistosoma mansoni egg antigen and were then injected intravenously with either Sephadex beads coated with PPD (Thl-type model) or SEA (Th2-type model). Cytokines and lesion morphology was performed on lungs removed from MIP-la knock-out and CCR2 knock-out mice 4 d after PPD or SEA beads were injected. 

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