Molecular sieve chromatography

Size exclusion HPLC has many other common names, such as gel permeation, gel filtration, steric exclusion, molecular sieve chromatography, or gel chromatography. These names all reflect the theoretical mode of action for this type... 

Gel chromatography or molecular sieve chromatography such as polyacrylamide gels... 

Morris, CJOR Morris, P, Molecular-Sieve Chromatography and Electrophoresis in Polyacrylamide Gels, Biochemical Journal 124, 517, 1971. 

Hjerten, S. and Mosbach, R., Molecular sieve chromatography of proteins on columns of cross-linked polyacrylamide, Anal. Biochem., 3, 109, 1962. 

This separation method is based on the molecular size of analytes. Analytes pass through porous stationary phase materials having different pore sizes, and molecular interactions between analytes and the stationary phase surface must be eliminated. A very strong solvent is therefore required in this system. This system is also called gel filtration liquid chromatography, gel-permeation liquid chromatography, or molecular sieve chromatography. This system is used to... 

Ackers, G. K., and T. E. Thompson Determination of stoichiometry and equilibrium constants for reversible associating systems by molecular sieve chromatography. Proc. Natl. Acad. Sci. 53, 342 349 (1965). 

Molecular exclusion and restricted diffusion processes in molecular sieve chromatography. Biochemistry 3, 723—730 (1964). 

First isolated from the conceptuses of sheep, IFN- is the major conceptus secretory protein responsible for signaling maternal recognition of pregnancy in mminants [11]. it is produced in large quantities (200 jig in 30 h from a day 16 conceptus culture). The protein was purified using a combination of anion exchange and molecular sieve chromatography. 

P.Andrews, Molecular Sieve Chromatography, Brit.Med.Bull., 22 109 1966. 

Maleimido groups are introduced into AChE by reaction of their primary amino groups with the jV-hydroxy-succinimide moiety of SMCC m neutral medium. This reaction is very similar to that used for incorporation of biotin molecules into proteins Prior to this reaction, the enzyme is treated with / /-ethyl maleimide in order to block any thiol groups. Finally, SMCC-AChE is purified by molecular sieve chromatography on a Biogel A 1.5 m column. 

G. Molecular Sieves Chromatography. It is a method of separation using chromatographic columns packed with "molecular sieves (Refs 58, 78 82a). Molecular sieves are solid materials possessing a very high sorption ability (for small molecules), because they... 

Size exclusion/molecular sieve chromatography Electron microscopy Ultracentrifugation Ultra nitration NMR spectroscopy Small-angle x-ray scattering Encapsulation of water-soluble markers... 

Size exclusion/molecular sieve chromatography Ultra Itration/dia lysis Ultracentrifugation Fluorescent probes Spin label EPR NMR probes Calorimetry Microelectrophoresis Zeta potential... 

Cassidy and Niro [13] have applied high-speed liquid chromatography combined with infrared spectroscopy to the analysis of polyoxyethylene surfactants and their decomposition products in industrial process waters. Molecular sieve chromatography... 

In GC this process has been used to separate fixed gases such as hydrogen, oxygen, nitrogen, methane, carbon monoxide, ethane, carbon dioxide, and ethylene5 and it has been called molecular sieve chromatography. The sieves are natural zeolites or synthetic materials of which the alkali metal aluminosilicates are typical. Table 3 lists the pore sizes of some commercial sieves. Newer sieves have been especially prepared from carbon Figure 3.5 shows a separation on a typical one, carbosieve II-S. 

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