Exclusion limit

Equation 12.33 defines the packing material s exclusion limit. 

In between the inclusion limit and the exclusion limit, each solute spends an amount of time in the pore space proportional to its size. The retention volume for a solute is... 

Another important application is for the determination of formula weights. Calibration curves of log(formula weight) versus Vj are prepared between the exclusion limit and inclusion limit (figure 12.36). Since the retention volume is, to some... 

Pipelines. The feasibility of pipeline transportation depends on the availability of very large quantities of compatible materials between locations with sufficient storage facilities. Thus, pipeline transportation is predominantly, but not exclusively, limited to the movement of hydrocarbons, many of which are raw materials in the production of petrochemicals. Although proprietary pipelines (qv), generally of short distances, ate not unusual, commercial petroleum pipelines are considered to be common carriers available to serve all customers who can tender sufficient quantities of acceptable liquids for transportation between terminals. 

An aqueous ethanol solution of acrylamide, 2,2 -methylenebisacrylamide as cross-linked agent and third acrylamide derivative, is dispersed in an n-alkane. Then three monomers are polymerized to spherical porous gels. The effect of the composition of the third monomer on the exclusion limits of the gel in size-exclusion chromatography has been investigated (82). 

Type Bead size djo (/xm) Size exclusion pore dimension (nm) Exclusion limit protein (M,) Fractionation range protein (/V ,) pH stability (long term/ short term)... 

The different pore sizes and exclusion limits of each TSK-GEL SW column will have a substantial effect on the resolution of a biomolecule mixture. G2000SW packing, which has the smallest pores, provides the best resolution for smaller proteins such as myoglobin and cytochrome c (16,900 and 12,400 Da, respectively, Rs = 1.01). Resolution of the proteins myoglobin... 

Larger particle sizes of each group are for 21.5 mm X 60 cm semipreparative and 55 or 108 mm X 60 cm preparative columns. Maximum separation range determined from estimated exclusion limits. 

Description Particie size (/im) Minimum theoreticai piates/30 cm Exclusion limit (poiystyrene moiecuiar weight) Standard flow rate (ml/min) Maximum flow rate (ml/min) Maximum pressure drop/30 cm (bar)... 

H type resins are available in different pore sizes. Examples of calibration curves for polystyrene standards are shown in Figs. 4.38 and 4.39. Other series of H type columns have similar calibration curves. Exclusion limits are listed in Tables 4.12-4.16. 

In-column solvents Column size (mm) Theoretical plate number Exclusion limit Poiystyrene PEG Particle size (/urn) Pore size (A) Flow Rate (ml/min) Maximum pressure (kgf/cm ) Maximum temperature (°C)... 

Three different types of columns packed with gels of different pore sizes are available. Columns should be selected that are suitable for the molecular weight range of specific samples, as each type has a different exclusion limit (Fig. 6.41, page 215). Bovine serum albumin (BSA), myoglobin, and lysozyme show good peak shapes using only 100 mM of sodium phosphate buffer as an eluent. There is no need to add any salt to the eluent to reduce the ionic interaction between protein and gel. 

The neutral hydrophilic surface and the wide range of pore diameters available for SynChropak GPC allow many compounds from small peptides to nucleic acids and other polymers to be analyzed. Table 10.2 lists the approximate exclusion limits for both linear and globular solutes. Although this information... 

Exclusion limits and practical operating ranges specified for PLgel individual pore size columns are summarized in Table 12.1. Table 12.1 illustrates the versatility of this family of columns. Pore volume can also be expressed as... 

Column type Exclusion limit (polystyrene equivalent) Operating range (polystyrene equivalent)... 

The main criterium for column selection is pore size distribution as it is desirable to have maximum pore volume for separation in the molecular weight range of interest. Having determined the upper molecular weight limit required, a column with a suitable exclusion limit should be selected. In the case of individual pore size columns, it is then a question of selecting other columns with complementary calibration curves to comprise a column set covering the re-... 

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