Separator Toyopearl

TABLE 4.20 Properties and Molecular Weight Separation Ranges for Toyopearl HW Resins... 

The hydrophilic surface characteristics and the chemical nature of the polymer backbone in Toyopearl HW resins are the same as for packings in TSK-GEL PW HPLC columns. Consequently, Toyopearl HW packings are ideal scaleup resins for analytical separation methods developed with TSK-GEL HPLC columns. Eigure 4.44 shows a protein mixture first analyzed on TSK-GEL G3000 SWxl and TSK-GEL G3000 PWxl columns, then purified with the same mobile-phase conditions in a preparative Toyopearl HW-55 column. The elution profile and resolution remained similar from the analytical separation on the TSK-GEL G3000 PWxl column to the process-scale Toyopearl column. Scaleup from TSK-GEL PW columns can be direct and more predictable with Toyopearl HW resins. 

Many operating variables, such as sample volume, flow rate, column length, and temperature, must be considered when performing any separation. The relative importance of these variables for Toyopearl HW-55F resin columns has been specifically evaluated. For example. Fig. 4.47 shows the relationship between column efficiency, or height equivalent of a theoretical plate (HETP),... 

Finally, temperature can have a profound effect on column performance. Tanaka et al. (48) studied the effects of temperature on the separation of hydrolyzed /3-cyclodextrin. In their studies, resolution increased with temperature on a Toyopearl HW-40S column. 

I. Separation of Low Molecular Weight Samples Using Toyopearl HW-40 Columns... 

Toyopearl HW-50S resin has been used to help isolate the ubiquitin-histone conjugate mH2A from the unicellular ciliated protozoan Tetrahymena pyriformis. Figure 4.49 shows the separation of mH2A from the histone, H2A. The sole difference between these two components is a small polypeptide, ubiquitin (approximately 8500 Da). The mH2A fraction was then further purified by HPLC on a Tosoh ODS-silica column (52). One of the many benefits... 

Toyopearl HW-75 resin, with pores larger than 1000 A, have been used in place of ultracentrifugation steps for the purification of plasmid DNA. Ultracentrifugation is a time-consuming process and requires expensive chemicals, such as cesium chloride. Toyopearl HW-75 resin provides superior separation performance for plasmid DNA and also provides high yields (54). 

Enzymatic preparation with predominant content of pectinesterase (obtained from Penicillium fellutanum culture liquid by isolation by acetone was purified. Primary enzymatic preparation was re — precipitated by three volumes of ethyl alcohol and centrifuged (6000 rev/min.) during 20 min. The obtained precipitate of partially purified pectinesterase preparation was dried in vacuum — desiccator. Sephadexes G — 50, G-75, G-lOO, G-200 "LKB" (Sweden) and Toyopearl HW-55 (Japan) were used for separation of enzymatic complex by gel — filtration. 

Ozawa, T., Separation of the components in black tea infusion by chromatography on Toyopearl. Agric. Biol. Chem., 46 1079, 1982. 

Crude GSE can be separated into distinct components from monomer-rich to oligomer- and polymer-rich fractions by solvent extraction followed by chromatography on Toyopearl resin. The pre-fractionation of the tannin crude extract by liquid-liquid extraction with ethyl acetate fractionates the monomers into the organic phase and improves the subsequent fractionation of oligomers and polymers on Toyopearl HW 40 resin. This fractionation technique is easy to operate and... 

Zaprometov. Effect of light on the for- CSl 49 mation of phenolic compounds in a suspension of a tea plant cells. Akad Nauk Gruz 1982 105 581-584-CS139 Ozawa, T. Separation of the components in black tea infusion by chromatography on toyopearl. Agr Biol Chem CS150 1982 46(4) 1079-1081. 

A number of techniques have been used for the preparative separation of flavonoids. These include HPLC, Diaion, Amberlite XAD-2 and XAD-7, and Fractogel TSK/Toyopearl HW-40 resins, gel filtration on Sephadex, and centrifugal partition chromatography (CPC). The choice of methods and strategies varies from research group to research group and depends often on the class of flavonoid studied. 

REE separation using AF-Chelate-650M Toyopearl resin (Tosoh Bioscience)... 

TSK Toyopearl is a spherical porous hydrophilic vinyl polymer it is generally thought that the polymer is poly(vinyl alcohol). TSK Toyopearl-IDA-Zr(IV) microspheres (IDA content 33 pmol/mL gel) were prepared for the separation and preconcentration of fluoride at ng/mL level in a flow system. 

It is the development of separation and identification techniques that makes the isolation and elucidation of the stilbenoids applicable. Silica gel is still the most commonly used material of the stationary phase. However, many other materials possessing better resolution or different isolation mechanisms have also been widely applied, e.g. Sephadex LH-20 [299 etc.], ODS Cis [208,299 etc.], MCI gel CHP20P, Toyopearl HW-40F [20,27,114,242,354], especially the former two. The combination use of the materials is efficient for the separation of the structures with high similarity and high polarity such as stilbene glucoside sulfates (34—43) [27]. 

FIGURE 26.1 (A) Effect of three fractions separated by Phenyl-Toyopearl column chroma-... 

On the other hand, the residue after the concentration by rotary evaporator was separated on normal phase gel adsorbents HP-20, Sephadex LH-20, and Toyopearl HW-40, to give 3 mg of a hatching stimulator. This material was active at 10 " g/mL. The activity of this stimulant was increased more than 100-fold by SylA and SylB. Syll had a striking synergistic activity, enhancing the hatching activity ca. 100,000-fold. 

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