Sensors recognition element

Binding of the analyte to the MIP is detected by the transducer and this results in an output signal. Typically, the system can be calibrated to provide quantitative information. MIPs have been used as sensor recognition elements for a variety of analytes using transduction methodologies based on... 

Other simulation experiments performed in our laboratory include design of MIP adsorbents for solid-phase extraction of atrazine [77], DDT, lindane, aflatoxin Bl, ochratoxin A, and tylosin (unpublished data) and the development of assay/ sensor recognition elements for biotin (unpublished data) and creatinine [78]. In all these cases, molecular modeling proved to be a useful tool for MIP design. It would... 

Micromonoliths used as sensor recognition elements provide a quick and easy fabrication scheme. One limitation to these monolith sensors was their capacity. The capacity of rod polymers was found to be very low and lower than LC columns packed with MIP particles. However, their ability to separate templates such as chloramphenicol from structurally similar analogues has been shown to be slightly better [47]. This may be an indication that while the number of viable imprinted sites is less, they are of similar selectivity. 

Optical sensors based on spectroscopy of guided waves have been demonstrated for detection and identification of numerous chemical and biological analytes. The choice of detection format for a particular application depends on the size of target analyte molecules, binding characteristics of available biomolecular recognition element, and range of analyte s concentrations to be measured. 

The type of matrix used for immobilization of the recognition element for bacterial cell detection is crucial to achieve high sensitivity. Two important conditions should be considered specifically for bacterial detection (1) the accessibility of the recognition elements in the immobilization matrix for bacteria binding on the sensor surface and (2) to obtain the binding of the analytes within the most sensitive region of the evanescent field, immediately adjacent to the sensor surface. 

Based on its structure, a sensor is an analytical device consisting of two main parts a (bio)chemical microzone (recognition element) that is brought into contact with the sample and closely associated (connected or integrated)... 

Figure 1.6 shows the major types of recognition elements and transducing systems used for implementing (bio)chemical sensing. A detailed discussion is provided in other sections of this chapter and the selected examples described throughout this book. Recently, Wolfbeis reported a systematic review of recognition elements based on enzymes, ion-carriers and molecular interactions used in optical sensors [5]. 

One very useful technical classification of sensors establishes two categories according to the relationship between the recognition element and the transducer. These two essential elements can be connected optically (e.g. 

The second group of properties is concerned with sensor operation in general and the peculiarities of the recognition element in particular. Ideally, a sensor should be fully reversible. Otherwise, it should lend itself readily to rapid, effective, reliable regeneration in order to be actually reusable. If... 

The flow-through sensors described in this Section comply essentially with the definition of biosensor. This word, like every term used to designate devices of scientific and popular note, has been the object of a number of definitions of both generic and specific scope. In a broad sense, a biosensor is any instrument or technique that measures biomolecules. In stricter terms, Rechnitz defines a biosensor as "a device that incorporates a biochemical or biological component as a molecular recognition element and yields an analytical signal in response to biomolecules" [10]. In between these two... 

A final example of a mass-sensitive MIP device is a B AW sensor for determination of phenacetin in human serum and urine (Tan, Peng, et al. 2001). A phenacetin imprinted polymer was synthesized and used as the artificial recognition element on a piezoelectric element. 

Hirsch T, Kettenberger H, Wolfbeis OS, Mirsky VM. A simple strategy for preparation of sensor arrays molecularly structured monolayers as recognition elements. Chem Commun 2003 432-433. 

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