Salmonella polysaccharides from

The method has also been applied to acetylated polysaccharides. Thus, whereas the D-mannopyranose residue in the acetylated lipo-polysaccharide from Salmonella typhi was found to be inert, that in Salmonella Strasbourg was oxidized, demonstrating that the former is a-D-linked and the latter /3-D-linked.154 The D-mannose residues in the acetylated lipopolysaccharide from Klebsiella O-group 3 were found resistant to oxidation, and are consequently a-D-linked."... 

An anti-2-acetamido-2-deoxy-D-mannose immunoglobulin, MOPC 406, is an IgA that binds73 polysaccharides from Salmonella weslaco and Escherichia coli 031. These polysaccharides are known to contain 2-acetamido-2-deoxy-D-mannose,83 and it has been shown that the specificity of this protein is directed towards /3-D-linked 2-acetamido-2-deoxy-D-mannopyranosyl residues.84 Inhibition studies revealed that the apparent affinity of the methyl /3-D-glycoside for the combining region of MOPC 406 is high. 

CDP-6-deoxy-D-xy/o-hexos-4-ulose (7b) serves as a precursor in the biosynthesis of CDP-3,6-dideoxyhexoses28,168 having the d-ribo (paratose, 3,6-dideoxy- D-glucose ), o-xylo (abequose, 3,6-dideoxy- D-galactose ), and d-arabino (tyvelose, 3,6-dideoxy- D-mannose ) configurations. These monosaccharides are characteristic components of O-specific polysaccharides from Salmonella and Yersinia pseudotuberculosis. 

The chemical-enzymic approach to the synthesis of modified polysaccharides presents a good prospect for the preparation of small quantities of these polymers, which may prove very useful for immunochemical studies. The approach is certainly not limited by the specific case of Salmonella polysaccharides 10-12, and may well be extended to other polymers. The first results from this group322 show that several analogs of O-specific polysaccharides (18) of Salmonella serogroups C2 and C3 may be prepared through this approach. 

Synthesis of f -D-(l,6) linked disaccharides of N-fatty acylated 2-amino-2-deoxy-D-glucose an approach to the lipid A component of the bacterial lipopolysaccharide. Carbohydr. Res., 88, C10-C13 Kochetkov, N.K., Dmitriev, B.A., Malysheva, N.N., Chernyak, A.Ya., Klimov, E.M., Bayramova, N.E., and Torgov, V.I. (1975). Synthesis of O-p-D-mannopyranosy l-( 1 - 0-0-ot -L-rhamnopyranosy l-( 1 -3)-D-galacto-pyranose, the trisaccharide repeating-unit of the O-specific polysaccharide from Salmonella anatum. Carbohydr. Res. 15, 283-290... 

Synthesis of the tetrasaccharide repeating-unit of the O-specific polysaccharide from Salmonella senftenberg. Carbohydr. Res. 5 1, 269-27 1... 

N. K. Kochetkov, V. L. Torgov, N. N. Malysheva, and A. S. Shashkov, Synthesis of the pentasaccharide repeating unit of the O-specihc polysaccharide from Salmonella Strasbourg, Tetrahedron, 36 (1980) 1099-1105. 

Scheme 5 Derived structure of 0-polysaccharide from Salmonella riogrande 0 40. GalNAc, W-acetylgalactosamine Man, mannose Glc, glucose GIcNAc, W-acetylglucosamine.

A constit. of nucleic acids, several coenzymes and bacterial polysaccharides from Salmonella. Also occurs in plant glycosides and free in plants. Obt. comly. from yeast, or by redn. of Ribonic acid, R-128 which can be obt. by oxidn. then epimerisation of Arabinose, A-850. Inexpensive starting material for chiral synth. Hygroscopic. Mp 95° (87°). [a]o -21.5 -19.5 (H2O). p Tai 12.22(25°). Sweet taste, sweetness = 0.33 x sucrose. 

Among the tetra- and penta-saccharides to be synthesized were (53)/ (54) (the repeating-unit of the O-specific polysaccharide from Salmonella senftenberg), a derivative of (55), (56) (in connection with immunological studies of the blood-group substances), and (57) (one of the core oligosaccharides of human blood-group substances). ... 

D-Mannosyl oligopeptides derived from ovalbumin and the cell wall polysaccharide from Salmonella suis, and containing terminal 2-acetamido-2-deoxy-D-glucosyl residues, are effective inhibitors of binding of the toxin to the cells. A disulphide-linked conjugate of concanavalin A and fragment A from trypsinized diphtheria toxin has been synthesized and shown to be a toxin for HeLa, Chinese hamster ovary and SV3T3(murine) cells. ... 

We are greatly indebted to S. J. Angyal, J. E. Courtois, G. E. McCasland, M. Nakajima, and T. Postemak for gifts of the cyclohexanepentols mentioned, and to A. M. Staub and G. Bagdian who provided us with the polysaccharide material from Salmonella typhimurium. 

KDO appears to be unique to Gram-negative bacteria. In the LPS that have been studied, KDO residues are situated at the reducing ends of the polysaccharide domains, linking them, by ketosidic bonds, to the fatty-acid-substituted 2-amino-2-deoxy-D-glucosyl disaccharides referred to as lipid A. Fig. 2 is a block diagram indicating the location of KDO in the LPS from Salmonella. 

It is prepared from Vi capsular polysaccharide of Salmonella typhi. Immunity develops 7 to 15 days after injection and protection lasts for three years. 

As may be seen from Table I (see p. 327), MOPC 384 precipitates with the lipopolysaccharides from Proteus mirabilis sp2, Salmonella tranaroa, Escherichia coli 070, and Salmonella telaviv.7s Precipitation with the latter polysaccharide can be inhibited with methyl ct-D-galactopyranoside. No inhibition could be achieved by using p-aminophenyl 1-thio-a-D-galactopyranoside,85 but the specificity of this protein nevertheless appears to be for a-D-linked D-galac-topyranosyl residues. 

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