Saccharomyces cerevisiae repression

Yeasts contain a large number of different active and passive sugar-transport systems. The first of these to be cloned was the glucose-repressible, high-affinity passive glucose transporter of Saccharomyces cerevisiae, which is encoded by the SNF3 gene... 

Bergman, L.W., McClinton, D.C., Madden, S.L. Preis, L.H. (1986). Molecular analysis of the DNA sequences involved in the transcriptional regulation of the phosphate-repressible acid phosphatase gene (PH05) of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences (USA) 83, 6070-4. 

K. A. Bostian, J. M. Lemize and H. O. Halvorson (1983). Physiological control of repressible acid phosphatase gene transcripts in Saccharomyces cerevisiae. Mol. Cell Biol., 3, 839-853. 

Approximately 1 p% of wild-type or mutant pCLI plasmid DNA is used for transformation of yeast cells. Spheroplasts of Saccharomyces cerevisiae strain GRF 18023,24 are prepared following the protocol of Burgers and Percival25 with some modification. High copy number transformants are directly selected on minimal medium plates lacking leucine and containing 8% glucose (to repress lysozyme expression) and 1 M sorbitol as an osmotic stabilizer. 

The NADH dehydrogenase of yeast is of considerable interest because in Saccharomyces cerevisiae and Saccharomyces carlsbergensis coupling site 1 is absent, whereas in Candida utilis its existence depends on the growth phase of the cells and can be altered by adaptations to culture conditions and by catabolite repression. 

Culbertson, M.R., Donahue, T.F., and Henry, S.A., 1976a, Control of inositol biosynthesis in Saccharomyces cerevisiae Properties of a repressible enzyme system in extracts of wild type (.Ino+) cells. J. Bacteriol. 126 232-242. 

Lamping, E., Paltauf, F., Henry, S.A., and Kohlwein, S.D., 1995, Isolation and characterization of a mutant of Saccharomyces cerevisiae with pleiotropic deficiencies in transcriptional activation and repression. Genetics 137 55-65. 

Krebs cycle and the constituents of respiratory chains are repressed (Gancedo 1992 Polakis et al. 1965 Barnett and Entian 2005). Therefore, under wine fermentation conditions, Saccharomyces cerevisiae can only ferment sugars. Saccharomyces cerevisiae can only use respiration when the sugar concentration is really low and when oxygen is present in the medium. These conditions are used for the industrial production of selected dry yeast. 

Meijer, M.M.C., Boonstra, J., Verkleij, A.J., Veriips, C.T. (1998) Glucose repression in Saccharomyces cerevisiae is related to the glucose concentration rather than the glucose flux. J. Biol. Chem., 273, 24102-24107. 

Thibon, C., Marullo, R, Claisse, O., Cullin, C., Dubourdieu, D., Tominaga, T. (2008b). Nitrogen catabolic repression controls the release of volatile thiols by Saccharomyces cerevisiae during wine fermentation. FEMS Yeast Research. (DOI 10 1111/j. 1567-1364.2008.00381). 

Boy-Marcotte, E., Perrot, M., Bussereau, F., Boucherie, H., and Jacquet, M. 1998. Msn2p and Msn4p control a large number of genes induced at the diauxic transition which are repressed by cyclic AMP in Saccharomyces cerevisiae. J. Bacteriol. 180,1044—1052. 

Lowry, C. V. and Zitomer, R. S. 1988. ROXl encodes a heme-induced repression factor regulating ANBl and CYC7 of Saccharomyces cerevisiae. Mol. Cell. Biol, 8, 4651-4658. 

Cunningham TS, Andhare R, Cooper TG (2000) Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gatlp and Ure2p production in Saccharomyces cerevisiae. J Biol Chem 275 14408-14414... 

Mitchell, A.P., and Magasanik, B. (1984). Regulation of glutamine-repressible gene products by the GLNB function in Saccharomyces cerevisiae. Mol. Cell. Biol. 4, 2758-2766. 

Nakagawa, Y., Sugioka, S., Kaneko, Y. and Harashima, S. 02R, a novel regulatory element mediating Roxlp-independent 0(2) and unsaturated fatty acid repression of OLEl in Saccharomyces cerevisiae. J Bacterial, 183 (2001) 745-751. 

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