Saccharomyces cerevisiae gene disruption

Burns, N., Grimwade, B., Ross-Macdonald, P., Choi, E.-Y., Finberg, K., Roeder, G., and Snyder, M. (1994). Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8, 1087—1105. 

Tsukiyama, T., Becker, P.B., and Wu, C. (1994) ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor. Nature 367, 525-532. Laurent, B.C., Yang, X., and Carlson, M. (1992) An essential Saccharomyces cerevisiae gene homologous to SNF2 encodes a helicase-related protein in a new family. Mol. Cell Biol. 12, 1893-1902. 

Sutter, T.R. and J.C. Loper (1989). Disruption of the Saccharomyces cerevisiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazole. Biochem. Biophys. Res. Commun. 160, 1257—1266. 

Lin SJ, Defossez PA, Guarente L (2000) Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science 289 2126—2128 Luo J, Nikolaev AY, Imai S, Chen D, Su F, Shiloh A, Guarente L, Gu W (2001) Negative control of p53 by Sir2alpha promotes cell survival under stress. Cell 107 137-148 Mabley JG, Suarez-Pinzon WL, Hasko G, Salzman AL, Rabinovitch A, Kun E, Szabo C (2001) Inhibition of poly (ADP-ribose) synthetase by gene disruption or inhibition with 5-iodo-6-amino-l,2-benzopyrone protects mice from multiple-low-dose-streptozotocin-induced diabetes. Br J Pharmacol 133 909-919... 

Esser, K., B. Scholle, and G. Michaelis (1999). Disruption of six open reading frames on chromosome X of Saccharomyces cerevisiae reveals a cluster of four essential genes. Yeast 15, 921-933. 

The existence of a mitochondrial pathway for de novo fatty acid synthesis was first reported 50 years ago, when it was generally assumed that fatty acid synthesis proceeded by reversal of the mitochondrial pathway for fatty acid P-oxidation (F. Lynen, 1957). However, the discovery of the cytosolic malonyl-CoA pathway (R.O. Brady, 1958 S.J Wakil, 1958) casted doubt on these claims and interest in this system waned until the discovery that mitochondria of both Neurospora crassa and Saccharomyces cerevisiae contain nuclear-encoded mitochondrial proteins that function as a type II FAS system. Disruption of the genes encoding these enzymes in both N. crassa and S. cerevisiae produces respiratory-deficient phenotypes and in S. cerevisiae cellular lipoic acid is reduced to less than 10% of that of the wild-type strain (R. Schneider, 1995 E. Schweizer, 1997). These observations suggested that in fungi one of the roles of this pathway might be to generate the lipoyl moieties required for mitochondrial function. 

Garrett, S. Broach, J. Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase. Genes Dev., 3, 1336-1348 (1989)... 

De Virgilio C, Burckert N, Bell W, et al. (1993). Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur. J. Biochem. 212 315-323. 

Kerry-Wiliams S.M., Gilbert S.C., Evans L.R. and Ballance D.J. 1998. Disruption of the Saccharomyces cerevisiae YAP3 gene reduces the proteolytic degradation of secreted recombinant human serum albumin. Yeast, 14, 161. 

Suzuki T, Sakamoto T, Sugiyama M, Ishida N, Kambe H, Obata S, Kaneko Y, Takahashi H, Harashima S. (2013). Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae. J Biosci Bioeng, 115(5), 467 74. 

Puccia, R., B. Grondin, and A. Herscovics, Disruption of the processing a-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae, Biochem. J., 1993, 290, 21-26. 

Arima, H., Kinoshita, T., Ibrahim, H.R., Azakami, H., Kato, A. Enhanced Secretion of Hydrophobic Peptide Fused Lysozyme by the Introduction of N-glycosylation Signal and the Disruption of Calnexin Gene in Saccharomyces cerevisiae FEBS Lett. 1998 440, 89-92. 

---