Saccharomyces cerevisae

Materials. Soluble PGG Glucan (Betafectin, Alpha-Beta Technology, Worcester, MA) was prepared from Saccharomyces cerevisae strain R4 cells (14). 

Heiskenskjold A process for making an animal feed by growing the yeast Saccharomyces cerevisae on the waste liquor from paper manufacture by sulfite pulping. Developed in Finland in 1936. 

Several yeast species have been engineered for heterologous protein production but the most commonly used for these purposes are the baker s yeast Saccharomyces cerevisae and the methylotropic yeast Pichia pastoris. Yeast systems utilize both integrated and extrachro-mosomal (non-integrated) vectors. 

Marrot, L. and Agapakis-Causse, C. (2000) Differences in the photogenotoxic potential of two fluoroquinolones as shown in diploid yeast strain (Saccharomyces cerevisae) and supercoiled plasmid DNA. Mutation Research, 468, 1-9. 

Saccharomyces cerevisae, DNA repair-deficient strains, differential toxicity... 

Prepare 1% yeast extract (Saccharomyces cerevisae), 1% peptone, 2% glucose and 1% agar (by weight). 

Kadowaki, T., Chen, S., Hitomi, M., Jacobs, E., Kumagai, C., Liang, S. et al. (1994) Isolation and characterization of Saccharomyces cerevisae mRNA transport-defective (mtr) mutants. J. Cell Biol, 126, 649-659. 

Neville, M. and Rosbash, M. (1999) The NES-Crmlp export pathway is not a major mRNA export route in Saccharomyces cerevisae. EMBO J., 18, 3746-3756. 

Fig. 3.16.2. Effect of additives on survival of Saccharomyces cerevisae CBS 1171 (SC 1171) after freeze-drying. CFU, colony-forming units A, CFU/mL before freeze-drying O, yeast suspension without additives 1, maltose 2, tre-...

The yeast Saccharomyces cerevisae is well known as a host for the expression of heterologous proteins. It is also exceptionally useful for nutrient-dependent viability screening, and is even more versatile than E. coli. Examples of the myriad ways in which genes encoding heterologous proteins can be functionally expressed in yeast for HTS are readily available [13,36-39], However, few applications for antiparasitic drug discovery have been described. 

Saccharomyces cerevisae (XV185-14C, D7, RM52, D6, D5, D6-1) Gene mutation - - Parry et al. 1985... 

C. D. Castro, A. P. Koretsky and M. M. Domach (1999). NMR-observed phosphate trafficking and polyphosphate dynamics in wild-type and vphl-1 mutant Saccharomyces cerevisae in response to stresses. Biotechnol. Prog., 15, 65-73. 

In principle, the same carbohydrates and their degradation products formed after hydrolysis of wood can be recovered from sulfite spent liquors. However, this requires complicated and expensive separation techniques. The industrial use of sulfite spent liquor components is mainly limited to fermentation processes. The most common product is ethyl alcohol which is produced from hexose sugars by yeast (Saccharomyces cerevisae) and separated from the mixture by distillation. Even the carbon dioxide formed in the process can be recovered. Other fermentation products, including acetone, n-butanol, and lactic acid, can be produced by certain microorganisms. Because some contaminants, for example, sulfur dioxide, inhibit the growth of the yeast, they must be removed from the liquor prior to the fermentation. 

Sulfur dioxide produced weak increases in micronuclei after activation in a plant assay Tradescantia spp.). Mammalian (cow, ewe) oocytes exposed in vitro had higher levels of chromosome aberrations when exposed to SO2 without metabolic activation. The Saccharomyces cerevisae yeast test for gene mutations was also positive without activation. 

Enzymes in their natural environment have been used as "solid reagents" and Saccharomyces cerevisae is a good example [118]. Enzyme cartridges have also been used, as demonstrated by the determination of glucose in plasma using flow injection manifolds with spectrophotometric and chemiluminometric detection and an in-line immobilised enzyme coil [119]. The coil consisted of glucose oxidase covalently bound to the inner surface of a nylon tube (25 cm long, 1.0 mm i.d.) which was coiled into a cylindrical acrylic exoskeleton. 

E. Kmiec, In vitro and in vivo nucleotide exchange directed by chimeric RNA/DNA oligonucleotides in saccharomyces cerevisae, Mol. Microbiol. 2001, 40, 857-868. 

E. Enolase from Saccharomyces cerevisae (Baker s Yeast)... 

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