Yeast Pichia pastoris

Trimble, R.B., Atkinson, P.H., Tschopp, J.F. et al. (1991) Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris. The Journal of Biological Chemistry, 266 (34), 22807-22817. 

Cereghino, J.L. and Cregg, J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24 (1), 45-66. 

Lin-Cereghino, J., Wong, W.W., Xiong, S. et al. (2005) Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques, 38 (1), 44, 46, 48. 

Dienys, G., Jarmalavicius, S., Budriene, S. et al. (2003) Alcohol oxidase from the yeast Pichia pastoris — a potential catalyst for organic synthesis. Journal of Molecular Catalysis B-Enzymatic, 21 (1-2), 47—49. 

Vuorela, A., Myllyharju, J., Nissi, R., Pihlajaniemi, T. and Kivirikko, K.I. (1997) Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast Pichia pastoris. formation of a stable enzyme tetramer requires coexpression... 

Doring, F., S. Theis, and H. Daniel. Expression and functional characterization of the mammalian intestinal peptide transporter PepTl in the methylotropic yeast Pichia pastoris. Biochem. Biophys. Res. Commun. 1997, 232, 656-662. 

Pakkanen, O., Hamalainen, E. R., Kivirikko, K. I., and Myllyharju.J. (2003). Assembly of stable human type I and III collagen molecules from hydroxylated recombinant chains in the yeast Pichia pastoris. Effect of an engineered C-terminal oligomerization domain foldon./. Biol. Chem. 278, 32478-32483. 

Several yeast species have been engineered for heterologous protein production but the most commonly used for these purposes are the baker s yeast Saccharomyces cerevisae and the methylotropic yeast Pichia pastoris. Yeast systems utilize both integrated and extrachro-mosomal (non-integrated) vectors. 

Eukaryotic expression yeast Pichia pastoris] Rapid growth with ease of scale-up and Glycosylated product differs from... 

Boettner, M., Prinz, B., Holz, C., Stahl, U. and Lang, C. (2002). High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J. Biotechnol 99,51-62. 

The enantioselective hydrolysis of the key industrial starting compound methyl benzoate 41 was recently reported using a Candida rugosa isoenzyme LIP1 expressed in the methylotrophic yeast Pichia pastoris affording /-menthol 28 with > 99% ee. 69... 

Su, W, Mertens, J. A., Kanamaru, K., Campbell, W. H., and Crawford, N. M., 1999, Analysis of wild-type and mutant plant nitote reductase expressed in the methylod ophic yeast Pichia pastoris. Plant Physiol. 115 1135nll43. 

The first crystal structure of CKX, namely ZmCKXl, was published in 2004 [147], Fig. (4A). So far it is the only protein from the number of different enzymes and receptors acting specifically on cytokinins, where the structure has been solved. The crystals of ZmCKXl, expressed in the yeast Pichia pastoris, were crystallized by a sitting drop vapor diffusion method and grown in 100 mM sodium acetate (pH 4.6), 200 mM ammonium sulfate, and 12% (w/v) PEG 5.000 monomethylether. Crystals belonged to the P492i2 space group. 

Cai, J. and Gros, P. (2003) Overexpression, purification, and fimctional characterization of ATP-binding cassette transporters in the yeast, Pichia pastoris. 

FREYRE, F.M., et al.. Very high level expression of an anti-CEA single-chain Fv antibody fragment in the methylothrophic yeast Pichia pastoris, J. Biotechnol. 76 (2000) 157-163. 

Lin Cereghino J L, Cregg J M (2000). Heterologous protein expression in the methy-lotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 45-66. 

Lin Cereghino G P, Lin Cereghino J, Ilgen C, Cregg J M (2002). Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13 329-332. 

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