Rodent feed

Based on tests with laboratory animals, aniline may cause cancer. The National Cancer Institute (NCI) and the Chemical Industry Institute of Toxicology (CUT) conducted lifetime rodent feeding studies, and both studies found tumors of the spleen at high dosage (100 —300 mg/kg pet day of aniline chloride). CUT found no tumors at the 10—30 mg/kg per day feeding rates. The latter value is equivalent to a human 8-h inhalation level of 17—50 ppm aniline vapor. In a short term (10-d) inhalation toxicity test by Du Pont, a no-effect level of 17 ppm aniline vapor was found for rats. At high levels (47—87 ppm), there were blood-related effects which were largely reversible within a 13-d recovery period (70). 

In microtine rodents, feed aversion and toxicant avoidance were the most significant behaviors elicited by feed tainted with paraquat (Linder and Richmond 1990). 

ANTU was not carcinogenic in rodent feeding studies. Cases of bladder tumors among rat catchers exposed to ANTU have been attributed to P-naphthylamine, a manufacturing impurity of ANTU. In bacterial assays ANTU induced mutations. 

Holcomb, M., Thompson, Jr., H. C., and Hankins, L. J. 1993. Analysis of fumonisin B1 in Rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection, J. Agricul. Food Chem., 41, 764-767. 

One way of improving SFE efficiency is by using a more suitable SF to extract the target analytes. Unfortunately, the choice of fluids other than CO2 is restricted by the desire to have reasonable critical parameter values and costs, chemical inertness, low toxicity, and little environmental impact. The use of supercritical N2O has proved to increase the extraction efficiency for a halo-genated aromatic phenoxy derivative of an aliphatic alkane (HAPA) from a dog feed and for a halogenated aromatic phenoxy derivative of urea (HAU) from a rodent feed (72). For the extraction of alkaloids from Amaryllidaceae plants, different CO2 and N2O modifiers such as methanol, ethanol, NH3, and so forth were investigated, providing the supercritical N2O with a mixture of NH3 methanol as modifiers better recoveries than pure or modified supercritical CO2 (47). 

Lectins are another class of potential antinutrient soy proteins (Sharon Lis, 1972 Liener, 1974). The carbohydrate content and structure of soy lectins were determined (Lis Sharon, 1978) and consist of mannose and Af-acetyl-glucosamine at about 5% by weight. Soy lectin has MW 110 kD from four identical subunits. The soy lectins are partly responsible for weight loss in rodent feeding studies with raw soy protein. Soy lectins are relatively easy to heat denature compared to other legume lectins. Properly processed soy foods have little native lectin present. Soybean lectins are widely used in clinical studies because of their interaction with red blood cell surface features (Friedman Brandon, 2001). 

LG Rushing, WM Cooper, HC Thompson Jr. Simultaneous analysis of vitamins A and E in rodent feed by high-pressure liquid chromatography. J Agric Food Chem 39 296-299, 1991. 

The assessment of compositional equivalence, livestock nutritional performance, and safety in rodent feeding studies share many common features. All these studies involve test, control, and reference groups, a specified experimental design that involves randomization and replication, followed by data collection (measurements) and lastly, evaluation (statistics). The study plan for the compositional analysis of maize has been described in detail previously (11) and is summarized in Table 1. The maize test line (event NK603), the control line (a near isogenic parental hybrid), and reference lines (19 conventional, non-transgenic commercial hybrids) obtained from controlled field trials were included in the study. Field sites were located in two different geographic areas, the United States (U.S.) and the European Union (E.U.), over... 

Kidney Effects of NP on the kidney have been one of the most consistently reported toxicological effects in rodent feeding studies, though effects are dependent on dose and diet composition. For instance, in a 90-day feeding study with... 

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