RNA silencing

Voinnet O (2005) Induction and suppression of RNA silencing insights from viral infections, Nat Rev Genet 6 206-220... 

Deuschle, K., Chaudhuri, B., Okumoto, S., Lager, I., Lalonde, S. and Frommer, W. B. (2006). Rapid metabolism of glucose detected with FRET glucose nanosensors in epidermal cells and intact roots of Arabi-dopsis RNA-silencing mutants. Plant Cell 18, 2314-25. 

RNAi has had an important impact on the development of novel disease models in animals, and it is likely that siRNAs, the trigger molecules for RNA silencing, will become an invaluable tool for the treatment of genetic disorders. The rational design of siRNAs, the introduction of chemical modifications into siRNAs to improve their pharmacokinetic and pharmacodynamic properties for in vivo application with high specificity, and the development of efficient delivery system will foster the therapeutic application of RNAi in AD and other neurodegenerative disorders (413,417). 

Hutvagner G, Simard MJ (2008) Argonaute proteins key players in RNA silencing. Nat Rev Mol Cell Biol 9 22-32... 

Peters L, Meister G (2007) Argonaute proteins mediators of RNA silencing. Mol Cell 26 611-623... 

Ribozyme-based RNA silencing has also been developed for G. instesti-nalis, although the technique has not been widely applied in the field (Dan et al. 2000). 

Timmons, L., Tabara, H., Mello, C.C. and Fire, A.Z. (2003) Inducible systemic RNA silencing in Caenorhabditis elegans. Molecular Biology of the Cell 14, 2972-2983. 

Silhavy, D., Molnar, A., Lucioli, A., Szittya, G., Hornyik, C., Tavazza, M. and Burgyan, J. (2002) A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs. EMBO Journal 21, 3070-3080. 

Chemical Genomics Reviews and Protocols, edited by Edward D. Zanders, 2005 309 RNA Silencing Methods and Protocols, edited by Gordon Carmichael, 2005... 

Voinnet, O. (2001). RNA silencing as a plant immune system against viruses. Trends Genet. 17, 449-459. 

Small Interfering RNA (siRNA) Technology. siRNA, a class of 20-25 nucleotide-long RNA molecules, is used in RNA silencing, a method that allows one to knock down expression of genes or more specifically to degrade the target mRNAs, in a sequence-specific fashion (Chakraborty 2007 Kumar... 

Another emerging theme in biology that is envisioned to enrich metabolic engineering is RNA silencing. The ensuing discussion also illustrates how synthetic DNA technology will be central to the fruitful implementation of the principles of RNA silencing. 

Tijsterman M, Ketting R, Plasterk R (2002) The genetics of RNA silencing. Annu Rev Genet 36 489-519... 

A family of ribonucleases that is involved in RNA silencing or RNA interference. See Conrad, C. and Rauhut, R., Ribonuclease 111 new sense from nuisance, Int. J. Biochem. 34, 116-129, 2002. 

Aravin A, Tuschl T. Identification and characterization of small 43. RNAs involved in RNA silencing. FEES Lett. 2005 579(26) 5830-5840. 

Kawamoto H, Koide Y, Morita T, Aiba H. Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Mol. Microbiol. 2006 61(4) 1013-1022. 

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