Rhodococcus globerulus

Several rhodococci have attracted interest for their ability to degrade PCBs including an organism (Acinetobacter sp. strain P6) now assigned to Rhodococcus globerulus (Asturias... 

Asturias JA, KN Timmis (1993) Three different 2,3-dihydroxybiphenyl-l, 2-dioxygenase genes in the Gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6. J Bacteriol 175 4631-4640. 

Rhodococcus globerulus P6 Burkholderia cepacia LB 400 Pseudomonas pseudoalcaligenes KF 707 Pseudomonas sp. KKS 102 Sphingomonas paucimobilis SYK 6 Pseudomonas sp. CA 10 Escherichia coli C Escherichia coli Alcaligenes eutrophus IMP 222 Sphingomonas paucinwbilis SYK 6... 

Asturias lA, LD Eltis, M Prucha, KN Timmis (1994) Analysis of three 2,3 -dihydroxybiphenyl 1,2-dioxygenases found in Rhodococcus globerulus P6. J Biol Chem 269 7807-7815. 

Collectively, these conclusions are supported by the results of a study of 33 congeners using Acinetobacter sp. strain P6 (Rhodococcus globerulus) (Asturias and Timmis 1993). The extradiol... 

Chebrou H, Y Hurtubise, D Barriault, M Sylvestre (1999) Heterologous expression and cbaracterization of tbe pnrified oxygenase component of Rhodococcus globerulus P6 bipbenyl dioxygenase and of cbimeras derived from it. J Bacterial 181 4805-4811. 

Dye contaminated soil and sludge Acid Orange-7, yeast extract Consortium consisting of Aeromonas caviae, Proteus mirabilis and Rhodococcus globerulus [58]... 

Acid Orange 7 and many other dyes Bacterial consortium TJ-1 consisting of Aeromonas caviae, Proteus mirabilis, and Rhodococcus globerulus Decolorization of Acid Orange 7 was significantly higher with the consortium as compared to the individual strains. More than 90% decolorization could be achieved even at 200 mg L 1 within 16 h. The consortium also decolorized 15 other azo dyes individually as well as a simulated wastewater containing a mixture of all the 16 azo dyes [58]... 

Pseuomonas putida Rhodococcus globerulus (old name Coryne-bacterium MBl) 0.3-0.4 n.d. 2.5-3 3 Catechol, Benzoate, Ethanol, Sali- [122] cylate. Phenol, 2-Chlorobenzoate Catechol, 2,3-Dihydroxybiphenyl, [ 119,121 ] Phenol, Benzoate, Chlorobenzoate, PCB... 

It is also possible to determine other PAH with microbial sensors, as demonstrated for dibenzofuran with Pseudomonas sp. HH693 and Sphingomonas sp. RW1 (old designation Pseudomonas sp. RWl) [121] as well as for biphenyl using Rhodococcus globerulus (old designation Corynebacterium MBl and Pseudomonas putida) [118-122]. However, the signal for these analytes is very... 

The mean problem when measuring many aromatics and their halogenated derivatives is their hydrophobicity. For that reason it is desirable that these compounds be measured in non-polar organic solvents. It was demonstrated, that the response of a Rhodococcus globerulus (old name Corynebacterium MBl) containing biosensor to 2,3-dihydroxybiphenyl in decane or octane remained constant for up to 60 min [119]. The use of -hexane in a biosensor system based on Pseudomonas putida increased the sensitivity for phenol fivefold, the measurement frequency sixfold, and operational stabihty was 7 h [113]. 

Asturias, J.A., Eltis, J.D., Prucha, M. Timmis, K.N. (1994). Analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenase genes found in Rhodococcus globerulus P6. Journal of Biological Chemistry, 269, 7807-15. 

The racemate 135, an isomer of 127 (n= 1), has also been resolved into 136 and a ring-opened product 137 by a lactamase present in the whole cells of Rhodococcus globerulus at pH 7 for 24 h. These /3-amino acids have been used in the syntheses of potential medicinal chemicals <2003G248, 2004T717>. 

Asturias, J.A. Diaz, E. Timmis, K.N., The evolutionary relationship of biphenyl dioxygenase from Gram-positive Rhodococcus globerulus P6 to multicomponent dioxygenases from gramnegative bacteria Gene 1995, 156, 11-18. 

The initial steps in the degradation of biphenyls by Pseudomonas sp. strain LB400 (Mondello 1989), by P. pseudoalcaligenes strain KF707 (Taira et al. 1992), and by Rhodococcus globerulus (Asturias and Timmis 1993). 

In another study, Rhodococcus globerulus PWD8 was found to oxidize D-limonene regio- and enantioselectively via (-t-)-trans-carveol to (+)-carvone [192l... 

The synthesis of the leading candidate compound 47 in an anticancer program [79,80] required (S)-2-chloro-l-(3-chlorophenyl)ethanol 48 (Figure 16.13) as an intermediate. Other possible candidate compounds used analogs of the (S)-alcohol. From microbial screening of reduction of ketone 49 to the (S)-alcohol 48, two cultures, namely, Hansenula polymorpha SC13824 (73.8% EE) and Rhodococcus globerulus... 

Of interest is the fact that p-lactamases from Rhodococcus globerulus could be used by Uoyd [66] to hydrolyze enantiomers of P-lactams to yield cyclic P-amino acids with greater than 90% ee. p-Lactamases belong to the P-lactam-recognizing enzymes (BLREs) superfamily. Their active site is characterized by a Ser-Ser-Lys catalytic triad and an oxyanion hole [67], similar to amidase signature enzymes. 

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