RFLPs informative

Correct answer = A. ELISA assays are commonly used to detect production of proteins. Northern blots could detect mRNA production, but would riot guarantee that the protein was made. Southern blots, ASO probes, arid RFLP analysis could be used to study the DNA inserted into the goat, but would not give information about protein production. 

As pointed out in the introduction the cold acclimation capacity is a quantitative trait and work is just beginning to link such complex traits to molecular markers using restriction fragment length polymorphism (RFLP). Eventually, also, information about genes involved in environmental stress can be expected from these experiments. 

In conclusion, these results further open the door to the creation of numerous minisatellite probes to survey for VNTR loci. Such probes can be used not only for restriction fragment length polymorphism (RFLP) analyses of the many biological applications outlined in the introduction, but they can also be employed to screen cloned libraries with the ultimate goal of determining the sequences flanking VNTR loci. The latter information provides perhaps the most accurate and rapid means of utilizing the variation at such loci via the PCR analysis of alternate alleles. 

Informative RFLP markers permit the tracking of chromosomes in prior and subsequent generations. Note in Fig. 2 that each child has inherited an RFLP allele from each parent where identical-sized alleles have been inherited, there is usually an increased intensity of hybridization reflecting the difference in allele dosage. 

The knowledge of the 3.2 biUion base pair human sequence information and information on oiu genetic diversity are milestones in oiu imderstanding of the contribution of genes to many complex problems such as disease predisposition and diversity of drug response by different patients. The manifestations of genetic diversity within the human genome are mostly SNPs, RFLPs (which can be... 

RFLP analysis can only be successful if the DNA detected is from a species with a known RFLP pattern. For successful real-time PCR it is necessary to know from which species the DNA should be detected, because specific primers for each species have to be used. In contrast, by sequencing of PCR products and databank research (www.ncbi.nlm.nih.gov/BLAST/) no information about the species possibly present is necessary, and species with unknown restriction patterns can be determined. 

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