Isogenic lines

Isogenic lines of spring wheat differing in capacity for ABA accumulation... 

Table 3. Examples of characters with possible roles in drought resistance for which isogenic lines or isopopulations have been developed...

Iwashina T, Githiri SM, Benitez ER et al (2007) Analysis of flavonoids in flower petals of soybean near-isogenic lines for flower and pubescence color genes. J Hered 98 250-257... 

Maekawa, M. et al.. Differential responses to UV-B irradiation of three near isogenic lines carrying different purple leaf genes for anthocyanin accumulation in rice Oryza sativa L.), Breeding Sci., 51, 27, 2001. 

Brandao et al. also applied the bottom-up approach to compare seed-protein profiles of herbicide-tolerant soybean and its near-isogenic line (60). Some 2-DGE parameters such as the loaded mass of the proteins, the pH separation range, and manual/automatic image editing were optimized prior to the evaluation (Figure 2). Of the 10 proteins with at least 90% variation found to be differentially expressed between the GM soybean and its counterpart, 8 proteins were successfully identified by MALDI coupled to a quadrupole (Q)-TOF MS. 

Eight combinations (types 1-8, Table 10.12) of Wx-Al, Wx-Bl and Wx-Dl proteins are possible in common wheats.257 Near-isogenic lines of the eight types of wheats showed apparent amylose levels of 3% to 25%, as determined by the blue color of the amylose-iodine complex.266 The amylose level in wheat kernels increases positively with the level of waxy proteins.257,269 271 In the double nulls, the Wx-Al protein in type 5 wheat produces —3% less amylose than the Wx-Bl and Wx-Dl proteins (types 6 and 7, respectively). In the single nulls, compared to the wild type (type 1), absence of Wx-Bl (type 3) reduces the amylose content by —2% compared to —1% for the absence of either of the other two waxy proteins (types 2... 

Obihiro,Tsukuba and Nagoya, Japan Near-isogenic lines of hexaploid wheats were produced with all combinations of waxy proteins double nulls contained up to 6-9% less amylose compared with the wild-type 266... 

Materials and Methods. Spider mites used in these experiments were maintained in colonies on bean plants as outlined by Rizzieri et al. (22.) Three colonies were used in the experiment. Two originated from field-collected populations one (Res) which was continuously selected with dicofol and was homogeneously resistant to dicofol, and the second (Sus), which was not selected with dicofol and was homogeneously susceptible to dicofol. Each colony was started with animals collected from a single, different apple orchard. The third colony was a near-isogenic line composed of the susceptible colony genome into which the factor conferring dicofol resistance had been... 

Figure e shows results obtained from cages started with spider mites from the near-isogenic line, IsoR, and the Sus colony. 

Recent studies by Fisher et in analyzing 16 isogenic lines having independent alleles of the maize ae... 

Table 11.2 Mixograph Dough Development Time and Bake-Test Loaf Volume for Isogenic Lines Differing in HMW-GS...

Figure 11.7 Mixograph dough development time (top left), percent of flour pro-fein (fop righf), percenf of polymeric profein (boffom right), and percent of unex-fracfable polymeric profein (boffom leff) for near-isogenic lines of three varieties having either Glu-Dla (HMW-GS 2+12) or Glu-Dld (HMW-GS 5+10). (Gupta, R. B., and F. MacRitchie. 1994. Journal of Gereal Science 19 19-29.)...

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