Reporter luminescent bacteria

Environment Canada (1992a) Biological test method toxicity test using luminescent bacteria (Vibrio fisheri), Environmental Protection Publications, Environment Canada, Ottawa, EPS Report l/RM/24,... 

Environment Canada. 1992. Biological Test Method Toxicity Test Using Luminescent Bacteria (Photobacterium phosphoreum). Environment Canada, Environmental Protection, Conservation and Protection. Environmental Protection Series Report EPS l/RM/24, Ottawa, Canada. 

The Microtox acute toxicity test is based upon measuring metabolic inhibition using a standard suspension of luminescent bacteria. Since bioluminescence is a direct measure of metabolic activity in these wganisms, measuring the rate of light output is a simple way to measure metabolic inhibition (toxicity). The test system has been in use for over 10 years and is the subject of many reports, reviews and application studies as well as being... 

To control the sea surface pollution, essentially by urban sewage, a simple indicator obtained by counting the colonies of luminescent and non-luminescent bacteria developing on filters, a solid culture medium, was applied [312]. Results from a reference area and polluted sites, and their variations during the year are reported. This method allows to assess the pollution degree and the area of the polluted sites to give recommendations on cleaning. 

The New Zealand freshwater limpet Latia neritoides (Fig. 6.1.1) is the only known example of a freshwater luminous organism, with two possible exceptions certain species of luminous bacteria and the larvae of certain species of fireflies. The limpet inhabits shallow clear streams in the North Island of New Zealand, clinging to stones and rocks. Latia has a small oval-shaped shell (6-8 mm long), and secretes a luminous mucus that emits a greenish glow around the body only when disturbed the limpet does not show a spontaneous luminescence. The luminescence of Latia was first reported by Suter (1890) and further details including a positive luciferin-luciferase reaction were described by Bowden (1950). Both the luciferin and the luciferase have... 

The more than 6000 species show various mechanisms of defense against predators. In marine species, amphipod luminescence always seems to be a defensive response.88 In the freshwater species Hyalella azteca, a bioluminescence caused by bacteria was reported.89... 

Similarly to modification of enzymes, for application in biosensors also, luminescence-based microbial biosensors were reported with the use of genetically engineered bacteria for such important environmental applications as determination of polycyclic aromatic hydrocarbons,125 or for genotoxicity monitoring.126... 

Harvey, published in 1940 and 1941. The present report deals largely with the subsequent advances, with particular reference to the luminescence of bacteria. 

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