Report cell

Balaguer, R, Fenet, H., and Georget, V. et al. (2000). Reporter cell lines to monitor steroid and antisteroid potential of environmental samples. Ecotoxicology 9, 105-114. 

C. Kotts, E. Gilkerson, D. Trinh, G. Hawker, A. Chen, H. Gazzano-Santoro, Assay validation report Cell proliferation test for human growth hormone, Pharmacopeial Forum, 25(3), 8313 (1999). 

Balaguer P, Francois F, Comunale F, Fenet H, Boussioux AM, Pons M, Nicolas JC, Casellas C (1999) Reporter cell lines to study the estrogenic effects of xenoestrogens. Sci Total Environ 233 47-56... 

Ringerike, T. et al., Detection of immunotoxicity using T-cell based cytokine reporter cell lines ( Cell Chip ), Toxicology, 206, 257, 2005. 

Assays can be prepared with a reporter system containing, for example, the hrefly luciferase gene. The reporter cells are coupled to receptor genes. When a ligand binds to the receptor, a luminescent glow can be observed. In this way, the effects of the signaling events are evaluated. 

One manufacturer reported cell power densities of 4.3 w/cm at a cell voltage of 0.8 V with an advanced space application cell configuration incorporating a 50 pm thick electrolyte matrix. 

Scott, G.K,Marden, C.,Xu, F., Kirk, L. and Benz, C.C. (2002) Transcriptional repression of ErbB2 by histone deacetylase inhibitors detected by a genomically integrated ErbB2 promoter-reporting cell screen. Molecular Cancer Therapeutics,... 

Dimeric forms of ferulic and p-coumaric acids also occur in bound form within plant cell walls. The most common of these is didehydroferulic acid 23, which has been isolated from some plants of the Gramineae (53,54) and spinach cell cultures (7). While no such p-coumaric acid 2 derived dimer (i.e., C5-C5 linked) has ever been reported, cell walls of Lolium multiflorum contain another intriguing structural variation, namely 4,4 -dihydroxytruxillic acid 24 (5,55). 

We discussed reticulated foams earlier in this book. They appear to have many desirable properties of ideal scaffolds. Depending on the feedstock, the manufacturers can produce a wide variety of pore sizes. Foams made specifically for reticulation have very narrow pore size distributions. If we compare the reported cell size distribution with that of Zeltringer, we can illustrate the precision of the reticulated foam process in the context of scaffolds for cell growth. Caution is advised in reviewing the Figure 7.6 plot. It is qualitative and assumes a normal distribution for both systems. It estimates the Zeltringer data based on the published standard deviation. 

D-size cells on 2.25 O continuous test are reported. Cell (a) is a standard Leclanchd cell using a natural ore cell (b) is a HD Leclanche with electrolyte Mn02 cell (c) is a zinc chloride cell and cell (d) is an alkaline manganese primary unit. The differences at this current drain are striking the discharge capacities with a 0.9 V cut-off are in the ratio 0.12 0.24 0.55 1.00 for the four types. However, when less severe tests are considered, the disparities are less pronounced. Thus for the light industrial flashlight (LIF) test, the ratios are 0.40 0.61 0.96 1.00. 

Report cell population viabilities as measured by the Trypan blue exclusion test in the original and sorted cell samples. 

There are four major types of in vitro cytokine bioassay, according to the reaction elicited by the cytokine on the reporter cell. 

This can be characterized as direct cytotoxicity to the reporter cells [e.g., TNF assay (W15)]. Certain cytokines have also been shown to exert an antiproliferative effect on other cells in a dose-dependent manner [e.g., TGF-/1 assay (Y5)]. 

In one report, cell growth of chicken embryo spinal cord neurons was studied. These neuron cells have been deposited in a micromachined Si chip coated with a synthetic additive protein (polylysine). It was found that groups of neurites grew towards the channel (50 im wide) connecting between pits where the neurons were deposited [856]. 

Methanol/Acetone (1 1) stored at -20°C in a spark-proof freezer. Or 0.5% glutaraldehyde in PBS for fixing LacZ reporter cells. 

Cell Therapy—Technologies, Markets and Companies. Strategic Report Cell Therapy Technologies, Markets and Companies. Jain PharmaBiotech, 2007 l-690... 

Some of the highest numbers for the Hemiaulus-Richelia symbioses were reported in the western tropical North Atlantic (WTNA). Carpenter et al. (1999) observed an extensive bloom off the NE coast of South America in autumn of 1996. They reported cell densities from 10 to 10 Richelia Recently, in the same vicinity as the study of Carpenter et al. (1999), Foster et al. (2007) reported extremely high niJH gene copy (>10 copies L ) abundances (proxy for cell abundances) for Richelia associated H. hauckii and Rhizosolenia clevei. In addition, they found within the plume waters of the Amazon River runoff a positive correlation between salinity and the abundance of the H. hauckii-Richelia abundance (Foster et al, 2007). 

Emter R, Ellis G, Natsch A (2010) Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro. Toxicol Appl Pharmacol 245 281-290... 

Vanadate r GR-mediated transactivation Reporter cell system 105... 

Wunder F, Tersteegen A, Rebmann A et al (2005) Characterization of the first potent and selective PDE9 inhibitor using a cGMP reporter cell line. Mol Pharmacol 68 1775-1781... 

Figure 2.5. A schematic of a p-lactamase reporter cell line that is coupled through a seven transmembrane G-protein—coupled receptor whose second messenger signaling pathway operates through cyclic adenosine monophosphate (cAMP). The G-protein, Gas, activates adenyl cyclase, increasing the concentration of cAMP, which stimulates the production of p-lactamase through cAMP response element.

Figure 2.7. Demonstration of a j3-lactamase reporter cell line. In a final volume of 0.02 mL, 5000-10,000 cells were seeded overnight at 37 C before being stimulated with excess agonist or antagonist for 4 h. The resultant /3-lactamase activity was measured after a 1 -h incubation with CCF2 (AuroraBio-sciences) at the indicated wavelengths in an LJL Analyst fluorometer. (a) The unstimulated cels, (b) the cells incubated with agonist, and (c) cells treated with agonist and excess antagonist. See color insert.

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